文献类型：期刊文献

英文题名：Characterization of a myoinhibitory peptide and its role in neuroimmune regulation in Litopenaeus vannamei during Vibrio harveyi infection

作者：Huang, Yongxiong[1,2];Huang, Meiling[4];Tan, XuYan[1,2];Zhang, Zhiqiang[1,2];Cai, Jia[1,2,3];Lu, Yishan[1,2,3];Huang, Yu[1,2,3];Jian, Jichang[1,2,3]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Key Lab Control Dis Aquat Anim Guangdong Higher Ed, Fisheries Coll, Zhanjiang 524088, Peoples R China;[3]Guangdong Prov Engn Res Ctr Aquat Anim Hlth Assess, Shenzhen, Peoples R China;[4]Foshan Inst Agr Sci, Foshan 528251, Peoples R China

年份：2026

卷号：168

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:001612892300002)、、WOS

基金：This work was funded by the Modern Seed Industry Park for Whiteleg Shrimp of Guangdong Province (K22219) and Open Fund of Tianjin Key Lab of aquatic ecology and aquaculture (TJAE201506) .

语种：英文

外文关键词：Litopenaeus vannamei; Myoinhibitory peptide (MIP); Neuroimmune regulation; Vibrio harveyi

外文摘要：Myoinhibitory peptides (MIPs) are a conserved subfamily of RFamide neuropeptides with multiple physiological functions, including neuromodulation and regulation of muscle contraction. However, the immunological function of MIP in Litopenaeus vannamei remains poorly understood. In this study, an myoinhibitory peptide gene (Lv-MIP) was cloned and characterized in L. vannamei and its role in bacterial infection was investigated. The open reading frame of Lv-MIP encodes a 99-amino acid peptide that shows high sequence homology with MIPs from other crustaceans. Tissue distribution analysis revealed that Lv-MIP is highly expressed in the ventral nerve cord and brain. Following Vibrio harveyi infection, Lv-MIP expression was significantly downregulated. In vivo experiments demonstrated that exogenous administration of synthetic Lv-MIP during bacterial challenge significantly decreased shrimp survival and increased inflammation and oxidative stress while participating in the c-Jun signaling pathway. These findings suggest that Lv-MIP plays a role in the neuroendocrine-immune response in L. vannamei, possibly through the MIP-SPR-Jun signaling axis. This study provides novel insights into the immunological function of neuropeptides in crustaceans and lays a foundation for further research on neuroimmune regulation in invertebrates.