文献类型：期刊文献

英文题名：Characterization and transcriptomic analysis reveal high aluminum adsorption performance of extracellular polymeric substances produced by Staphylococcus epidermidis GXALA1

作者：Chen, Yinyan[1];Fang, Zhijia[1];Li, Yongbin[1];Iddrisu, Lukman[1];Deng, Qi[1];Sun, Lijun[1];Liao, Jianmeng[2];Gooneratne, Ravi[3]

机构：[1]Guangdong Ocean Univ, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Coll Food Sci & Technol,Guangdong Prov Engn Techno, Res Ctr Marine Food,Guangdong Prov Key Lab Aquat P, Zhanjiang 524088, Peoples R China;[2]Zhanjiang Inst Food & Drug Control, Zhanjiang 524022, Peoples R China;[3]Lincoln Univ, Dept Wine Food & Mol Biosci, Lincoln 7647, Canterbury, New Zealand

年份：2026

卷号：337

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:001640807300001)、、EI(收录号：20255019693330)、Scopus(收录号：2-s2.0-105024360642)、WOS

基金：This work was supported by the Natural Science Foundation of Guangdong Province (No. 2024A1515011711) , and the National Natu-ral Science Foundation of China (No. 32172215) .

语种：英文

外文关键词：Aluminum; Adsorption; Staphylococcus epidermidis; Polysaccharides; Extracellular polymeric substances (EPS); Transcriptomics

外文摘要：Aluminum (Al) pollutants pose a significant environmental and human health risk. Microorganisms and their extracellular polymeric substances (EPS) have superior potential in the bioremediation of metals in environments. Herein, Staphylococcus epidermidis GXALA1 was isolated from highly Al-polluted soil in China and showed excellent Al3+ adsorption capacity. The microscopic analyses and adsorption experiments showed that 80 % of Al3+ was adsorbed onto the surface of S. epidermidis GXALA1 through EPS, forming granular deposits. The EPS secreted from S. epidermidis GXALA1 also displayed a strong adsorptive capacity, with a high adsorption rate of 93.88 %. The Freundlich isotherm model provided a better fit for the Al3+ experimental data with a higher R2 of 0.957, and the pseudo-second-order model fitted the sorption kinetic processes with an adsorption capacity of 87.19 mg/g. Multispectral analyses showed that the oxygen-containing groups (C--O, C-O-C, C-O, and P--O) of polysaccharides and the humic acid-like substances derived from EPS were involved in Al3+ adsorption. Meanwhile, increased polysaccharides in EPS in response to Al3+ stress may assure the strong Al3+ adsorbability of S. epidermidis GXALA1. The transcriptome sequencing analysis showed that carbohydrate metabolism (KFV35_RS01275, zwf, and glmU, LFC (log2 fold change) = 2.26, 1.99, and 1.15, respectively), glycan biosynthesis and metabolism (KFV35_RS01570, mraY, and murF, LFC = 1.53, 2.07, and 2.78, respectively), and transmembrane transporter pathways (ABC and MFS: ptsG and ugpC, LFC = 1.67 and 2.57, respectively) were significantly up-regulated, which facilitated polysaccharide synthesis and abundant EPS secretion containing the groups (C--O, C-O-C, C-O, and P--O). This study offers novel perspectives on Al3+ biosorption for future applications.