文献类型：期刊文献

英文题名：Inhibitory activity of a novel antibacterial peptide AMPNT-6 from Bacillus subtilis against Vibrio parahaemolyticus in shrimp

作者：Xu, Defeng[1];Wang, Yaling[1];Sun, Lijun[1];Liu, Huanming[1];Li, Jianrong[2,3]

机构：[1]Guangdong Ocean Univ, Dept Food Qual & Safety, Sch Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Zhejiang Gongshang Univ, Food Safety Key Lab Zhejiang Prov, Coll Food Sci & Biotechnol, Hangzhou 310035, Zhejiang, Peoples R China;[3]Bohai Univ, Food Safety Key Lab Liaoning Prov, Jinzhou 121013, Peoples R China

年份：2013

卷号：30

期号：1

起止页码：58

外文期刊名：FOOD CONTROL

收录：SCI-EXPANDED(收录号：WOS:000311175500010)、、WOS

基金：The authors gratefully acknowledge the National Science Fund (No.30279987) and the National Key Technologies R & D Program of China during the 12th Five-Year Plan Period (Key technology of quality and safety control during aquatic product processing, No. 2012BAD29B06) for its financial support.

语种：英文

外文关键词：Bacillus subtilis; Antimicrobial peptide; V. parahaemolyticus; Shrimps

外文摘要：Vibrio parahaemolyticus is a major foodborne pathogen in marine products. Effective methods for reducing V. parahaemolyticus in seafood would reduce the likelihood of foodborne outbreaks of Vibrio. AMPNT-6 is a novel antibacterial peptide secreted by Bacillus subtilis NT-6 isolated from Natto, a Chinese traditional fermented-soybean paste. The objective of this study is to investigate the antibacterial activity of AMPNT-6 against V. parahaemolyticus. The disc diffusion experiment indicated that AMPNT-6 posed an evident inhibitory activity against V. parahaemolyticus with a diameter of 15.5 mm and the MIC was measured at 1.25 mg/mL. Compared to the control, the significant bactericidal activity was observed through the changes of V. parahaemolyticus growth curve and inactivation kinetics when the final concentration of AMPNT-6 in bacterial suspension was added to 1 and 2 MIC. Furthermore the significant reduction of population size of V. parahaemolyticus in shrimp matrix was also found when the final concentration of AMPNT-6 reached to 2 MIC. These obtained results indicate that AMPNT-6 can potentially be used as a natural inhibitor to control V. parahaemolyticus borne on shrimp matrix. (C) 2012 Elsevier Ltd. All rights reserved.