文献类型：期刊文献

英文题名：Simultaneous aerobic nitrogen and phosphate removal capability of novel salt-tolerant strain, Pseudomonas mendocina A4: Characterization, mechanism and application potential

作者：Shu, Hu[2];Ma, Yonghao[1,2];Lu, Huijie[1];Sun, Huiming[1];Zhao, Jichen[1];Ruan, Zhuohao[1];Zhou, Jiayi[2];Liu, Yuting[2];Liu, Fengkun[1,3];Xu, Jingxuan[1,3];Zheng, Yazhi[1,3];Guo, Hui[3];Chen, Qionghua[2];Huang, Wen[1,3,4]

机构：[1]Guangdong Acad Agr Sci, Inst Anim Sci, Collaborat Innovat Ctr Aquat Sci, Guangdong Key Lab Anim Breeding & Nutr, Guangzhou 510640, Peoples R China;[2]Guangzhou Univ, Sch Life Sci, Guangzhou 510006, Peoples R China;[3]Guangdong Ocean Univ, Fishery Coll, Zhanjiang 524088, Peoples R China;[4]Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Peoples R China

年份：2024

卷号：393

外文期刊名：BIORESOURCE TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:001125432400001)、、EI(收录号：20234815131558)、Scopus(收录号：2-s2.0-85177777281)、WOS

基金：This work was supported by Guangdong Academy of Agricultural Science Talent Program (Grant No. R2020YJ-QG001, R2022PY-QY008, and R2017YJ-YB3007) ; Innovation Fund project of Guangdong Acad- emy of Agricultural Sciences (Grant No. 202137 and 202138) .

语种：英文

外文关键词：Nitrogen metabolism; Aerobic phosphate removal; Metabolism enzymes; Immobilization

外文摘要：A salt-tolerant strain, Pseudomonas mendocina A4, was isolated from brackish-water ponds showing simultaneous heterotrophic nitrification-aerobic denitrification and phosphorus removal capability. The optimal conditions for nitrogen and phosphate removal of strain A4 were pH 7-8, carbon/nitrogen ratio 10, phosphorus/nitrogen ratio 0.2, temperature 30 degrees C, and salinity range of 0-5 % using sodium succinate as the carbon source. The nitrogen and phosphate removal efficiencies were 96-100 % and 88-96 % within 24 h, respectively. The nitrogen and phosphate removal processes were matched with the modified Gompertz model, and the underlying mechanisms were confirmed by the activities of key metabolic enzymes. Under 10 % salinity, the immobilization technology was employed to enhance the nitrogen and phosphate removal efficiencies of strain A4, achieving 87 % and 76 %, respectively. These findings highlight the potential application of strain A4 in both freshwater and marine culture wastewater treatment.