文献类型：期刊文献

中文题名：甲基睾酮与来曲唑对遗传雌性阿纳鲳鲹性逆转的诱导机制

英文题名：Induction Mechanism of Methyltestosterone and Letrozole on Sex Reversal in Genetically Female Trachinotus anak

作者：江星怡[1];周琪[1];王岩[1];郭煜文[1];王焘[1];陈华谱[1]

机构：[1]广东海洋大学水产学院,广东湛江524088

年份：2026

卷号：46

期号：3

起止页码：74

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：北大核心2023、、北大核心

基金：湛江市科技计划项目(2024E03007,2025R02104,2025A0301004);广东省级人才项目(2023TQ07A888)。

语种：中文

中文关键词：阿纳鲳鲹;性逆转;甲基睾酮;来曲唑;性激素;全转录组

外文关键词：Trachinotus anak;sex reversal;methyltestosterone;letrozole;sex hormones;transcriptome

中文摘要：【目的】探究甲基睾酮(MT)和来曲唑(LZ)两种外源激素对阿纳鲳鲹(Trachinotus anak)性腺组织学、性激素和转录组的影响,解析MT和LZ诱导阿纳鲳鲹性逆转的分子机制。【方法】将30日龄的阿纳鲳鲹幼鱼分别用LZ、MT混合饲料及普通饲料投喂至6月龄,通过Sanger测序鉴定性别,对阿纳鲳鲹进行性腺组织学观察、性激素水平测定、全转录组测序分析,并利用实时荧光定量PCR(qPCR)对差异表达基因进行验证。【结果】组织学及血清学分析显示,LZ和MT处理使遗传雌性阿纳鲳鲹发生表型性逆转,逆转率分别为6.7%和16.7%。与雄性、雌性对照比较,逆转个体血清中雌二醇和卵黄蛋白原水平显著下降(P<0.05),雄激素(睾酮和11酮睾酮)有升高趋势,但差异不显著(P>0.05)。转录组测序共获得198.99×109和185.41×106个碱基的高质量数据,分别鉴定出92603条mRNA、21141条lncRNA和大量miRNA。功能富集分析显示,大量差异基因关联于多种生殖相关通路,包括卵母细胞减数分裂、类固醇激素生物合成和Wnt信号通路等,尤其显著关联于卵母细胞减数分裂通路。成功构建聚焦于卵母细胞减数分裂通路的蛋白互作网络(PPI)和lncRNA/miRNA-mRNA共表达调控网络,筛选出ccne2、cul1、mad2a等关键靶基因以及MSTRG.15985.1、MSTRG.16453.2、MSTRG.22617.1等核心非编码RNA。转录组和qPCR结果显示,LZ和MT处理组与正常雌鱼相比,prg、ar、novel_miR_10表达上调,cdc20、ccnb、cdc23、MSTRG.67400.1、MSTRG.68354.4、MSTRG.77162.2、MSTRG.107194.8、novel_miR_657、novel_miR_589、novel_miR_323表达下调。【结论】MT与LZ均可有效诱导表型雄性化。LZ主要通过抑制芳香化酶活性,直接扰乱雌激素合成并影响卵母细胞减数分裂过程;而MT则更多依赖雄激素受体信号通路及下丘脑-垂体-性腺轴负反馈调控,间接重塑性腺内分泌环境。卵母细胞减数分裂通路为MT和LZ诱导性逆转的核心分子靶点,卵母细胞重塑在性别逆转过程中发挥重要作用。非编码RNA在性腺分化与性别重塑中有精细调控功能。MT的性逆转效率高于LZ,在规模化性别控制育种中有更高的应用潜力。

外文摘要：【Objective】This study aims to investigate the effects of 17α-methyltestosterone(MT)and letrozole(LZ)on gonadal histology,sex steroid hormones,and transcriptomic profiles in Trachinotus anak,and to elucidate the molecular mechanisms underlying hormone-induced sex reversal in T.anak.【Methods】Juvenile T.anak at 30 days post-hatching were fed with diets containing LZ,MT,or a control diet until 6 months of age.Gonadal histology,sex hormone levels,and whole-transcriptome sequencing were analyzed,and selected differentially expressed genes were validated by quantitative real-time PCR(qPCR).【Results】Histological and serological analysis showed that LZ and MT induced phenotypic sex reversal in genetic female T.anak,with reversal rates of 6.7%and 16.7%,respectively.Compared with the male and female controls,serum estradiol and vitellogenin levels in sex-reversed individuals decreased significantly(P<0.05),while androgens(testosterone and 11-ketotestosterone)showed an upward trend,but the differences were not significant(P>0.05).Transcriptome sequencing yielded clean data with 198.99×109 and 185.41×106 bases,identifying 92603 mRNAs,21141 lncRNAs,and numerous miRNAs.Functional analysis revealed that differentially expressed genes were primarily enriched in reproduction-related pathways,particularly the oocyte meiosis pathway.Based on this pathway,a protein-protein interaction network and lncRNA/miRNA-mRNA co-expression networks were constructed,identifying key target genes(ccne2,cul1,mad2a)and core ncRNAs(MSTRG.15985.1,MSTRG.16453.2,MSTRG.22617.1).Transcriptomic and qPCR results confirmed that pgr,ar,and novel_miR_10 were upregulated,while cdc20,ccnb,cdc23,and several ncRNAs(e.g.,MSTRG.67400.1,novel_miR_657)were downregulated compared to normal females.【Conclusion】Both MT and LZ effectively induce phenotypic masculinization in T.anak.LZ acts primarily by inhibiting aromatase to disrupt estrogen synthesis and oocyte meiosis,whereas MT relies on androgen receptor signaling pathway and HPG-axis negative feedback to alter the gonadal endocrine environment.The oocyte meiosis pathway serves as the core molecular target,highlighting the critical role of oocyte remodeling in sex reversal.Non-coding RNAs precisely regulate gonadal differentiation.Given its higher efficiency,MT holds greater potential for large-scale sex-control breeding.