文献类型：期刊文献

中文题名：华贵栉孔扇贝全脏器中糖胺聚糖的分离提取及其抗肿瘤活性研究

英文题名：Extraction and Isolation of Glycosaminoglycan from Chlamys Nobilis and Study on its Anti-tumour Effect

作者：金晓石[1];胡雪琼[1];吴红棉[1];范秀萍[1];钟敏[1]

机构：[1]广东海洋大学海洋食品研究所,广东湛江524025

年份：2007

卷号：23

期号：3

起止页码：36

中文期刊名：现代食品科技

外文期刊名：Modern Food Science and Technology

收录：CSTPCD、、Scopus

语种：中文

中文关键词：华贵栉孔扇贝;糖胺聚糖;人宫颈癌细胞;抑制作用

外文关键词：Chlamys nobilis, Glycosaminoglycan; Hela cell; inhibited effect

中文摘要：以湛江地区盛产的华贵栉孔扇贝全脏器为原料,经木瓜蛋白酶和枯草杆菌中性蛋白酶双酶水解、醇沉后得到华贵栉孔扇贝糖胺聚糖粗品,采用MTT法检测粗品体外对人宫颈癌细胞(Hela)活性的影响。实验结果表明:采用双酶水解法粗品得率为1.26%,总糖含量为41.4%,糖胺聚糖(GAG)含量为26.9%。粗品具有对Hela肿瘤细胞的生长显著性抑制作用(p<0.05)。

外文摘要：Glycosaminoglycan （GAG） from the whole viscera of Chlamys nobilis was achieved via enzymatic hydrolysis by papain and neutral proteinase and ethanol precipitation. Its anti-tumor activity was also examined by MTT colormetric assay. Results showed that the yield of crude product reached 1.26%, and the content of total sugar and GAG were 41.4% and 26.9%, respectively. Besides, it showed significant anti-tumor activity towards Hela cell （p〈0.05）.