文献类型：期刊文献

英文题名：Dermatopontin, a shell matrix protein gene from pearl oyster Pinctada martensii, participates in nacre formation

作者：Jiao, Yu[1];Wang, Huan[1];Du, Xiaodong[1];Zhao, Xiaoxia[1];Wang, Qingheng[1];Huang, Ronglian[1];Deng, Yuewen[1]

机构：[1]Guangdong Ocean Univ, Fishery Coll, Zhanjiang 524025, Guangdong, Peoples R China

年份：2012

卷号：425

期号：3

起止页码：679

外文期刊名：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

收录：SCI-EXPANDED(收录号：WOS:000308626200032)、、WOS

语种：英文

外文关键词：Dermatopontin; Shell matrix protein; RNA interference; Nacre

外文摘要：Dermatopontin (DPT) is identified as a major component of the shell matrix protein. However, its exact function in the shell formation remains obscure. In this study, we described the characteristic and function of DPT gene from Pinctada marrensii. DPT cDNA was 797 bp long, containing an open reading fragment (ORF) of 537 bp encoding a polypeptide of 178 amino acids with an estimated molecular mass of 21.4 kDa and theoretical isoelectric point of 5.97. The 5' untranslated region (UTR) was 11 bp and the 3'UTR was 249 with 18 bp poly (A) tail. In the peptide, there was a signal sequence, six potential phosphorylation sites, one glycosylation site and eight cysteine residues. Moreover, a sequence motif (D-R-X-W/F/Y-X-F/Y/I/L/M-X1-2-C) was contained and repeated itself three times in the entire sequence. DPT mRNA was constitutively expressed in all studied tissues with the most abundant mRNA in the mantle, which was nacre formation-related tissue. After decreasing DPT expression using RNA interference (RNAi) technology in the mantle, the nacreous layer showed a disordered growth; whereas the prismatic layer of the shells has no significant changes. These results suggested that DPT obtained in this study was a constitutive matrix protein and participated in nacre formation in P. martensii. (C) 2012 Published by Elsevier Inc.