文献类型：期刊文献

英文题名：Assessment of the juvenile vulnerability of symbiont-bearing giant clams to ocean acidification

作者：Li, Jun[1,2,3];Zhou, Yinyin[1,2,3];Qin, Yanpin[1,2,3];Wei, Jinkuan[1,2,3];Shigong, Pengyang[1,2,3];Ma, Haitao[1,2,3];Li, Yunqing[1,2,3];Yuan, Xiangcheng[1,2,3];Zhao, Liqiang[4];Yan, Hong[5];Zhang, Yuehuan[1,2,3];Yu, Ziniu[1,2,3]

机构：[1]Chinese Acad Sci, Innovat Acad South China Sea Ecol & Environm Engn, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol,Guangdong, Guangzhou 510301, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Guangzho, Guangzhou 510301, Peoples R China;[3]Chinese Acad Sci, Hainan Key Lab Trop Marine Biotechnol, Hainan Sanya Marine Ecosyst Natl Observat & Res S, Sanya Inst Oceanol, Sanya 572024, Peoples R China;[4]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[5]Chinese Acad Sci, Inst Earth Environm, State Key Lab Loess & Quaternary Geol, Xian 710061, Peoples R China

年份：2022

卷号：812

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：SCI-EXPANDED(收录号：WOS:000740223300017)、、EI(收录号：20215211387510)、Scopus(收录号：2-s2.0-85121582113)、WOS

基金：The authors would like to thank the editor and three reviewers for their assistance in the manuscript revision process. This work was supported by the National Key Research and Development Program of China (2020YFD0901102; 2018YFD0901400); the Chinese Ministry of Science and Technology through the National Science Foundation of China (31872566; 31702340; 32002387); Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (GML2019ZD0402); Guangdong Provincial Key R&D Programme (2021B0202020003); Haina Provincial Key R&D Programme (ZDYF2021XDNY135, ZDYF2021XDNY183); Guangdong Basic and Applied Basic Research Foundation (2021A1515011181); Guangxi innovation-driven development program (No. AA19254032); the Network Service Local Plan STS of the Chinese Academy of Sciences (KFJ-STSQYZD-158); the Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences (ISEE2018PY01; ISEE2018ZD02); the Open Foundation of the State Key Laboratory of Loess and Quaternary Geology (SKLLQG1813; SKLLQG1918); China Agriculture Research System ofMOF andMARA; and the Science and Technology Planning Project of Guangdong Province, China (2020B1212060058).

语种：英文

外文关键词：Ocean acidification; Calcification; Symbiosis; RNA-seq; Tridacna spp

外文摘要：Ocean acidification (OA) severely affects marine bivalves, especially their calcification processes. However, very little is known about the fate of symbiont-bearing giant clams in the acidified oceans, which hinders our ability to develop strategies to protect this ecologically and economically important group in coral reef ecosystems. Here, we explored the integrated juvenile responses of fluted giant clam Tridacna squamosa (Lamarck, 1819) to acidified seawater at different levels of biological organization. Our results revealed that OA did not cause a significant reduction in survival and shell growth performance, indicating that T. squamosa juveniles are tolerated to moderate acidification. Yet, significantly reduced net calcification rate demonstrated the calcifying physiology sensitivity to OA, in line with significant declines in symbiont photosynthetic yield and zooxanthellae density which in turn lowered the amount of energy supply for energetically expensive calcification processes. Subsequent transcriptome sequencing and comparative analysis of differentially expressed genes revealed that the regulation of calcification processes, such as transport of calcification substrates, acid-base regulation, synthesis of organic matrix in the calcifying fluid, as well as metabolic depression were the major response to OA. Taken together, the integration of physiological and molecular responses can provide a comprehensive understanding of how the early life history stages of giant clams respond to OA and make an important leap forward in assessing their fate under future ocean conditions.