文献类型：期刊文献

英文题名：Purification and identification of an ACE inhibitory peptide from the peptic hydrolysate of Acetes chinensis and its antihypertensive effects in spontaneously hypertensive rats

作者：Cao, Wenhong[1];Zhang, Chaohua[1];Hong, Pengzhi[1];Ji, Hongwu[1];Hao, Jiming[1]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524025, Guangdong, Peoples R China

年份：2010

卷号：45

期号：5

起止页码：959

外文期刊名：INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000276606900014)、、WOS

基金：This work was supported by funds from the Science &Technology Exploration Plan Research Funding of Guangdong Ocean University (No. 0912047).

语种：英文

外文关键词：Acetes chinensis; angiotensin I-converting enzyme inhibitory peptide; antihypertensive effect; enzymatic hydrolysis

外文摘要：P>Acetes chinensis is a marine shrimp found in the coastal waters of China. The shrimp was hydrolysed by pepsin to prepare hydrolysates with angiotensin I-converting enzyme (ACE) inhibitory activity. The hydrolysate with the highest ACE inhibitory activity resulted from a 3-5 h incubation at 45 degrees C and pH 2.5 with pepsin. Gel filtration and RP-HPLC were used to separate ACE inhibitory peptides from the hydrolysate. The gel filtration fraction of the hydrolysate with a molecular weight range from 1320 Da to 311 Da exerted the highest ACE inhibition activity. This fraction was separated by RP-HPLC into fifteen fractions, of which fraction F9 showed 92.7% of the ACE inhibition activity. Its peptide sequence was determined to be Leu-His-Pro. It showed a potent antihypertensive activity in spontaneously hypertensive rats. The results suggested that this peptide may be a potent ACE inhibitor which might be developed into a healthy food to lower blood pressure.