文献类型：期刊文献

英文题名：Protective mechanism of tea polyphenols against muscle quality deterioration of shrimp (Penaeus vannamei) induced by aflatoxin B-1

作者：Huang, Zhanrui[1,2];Sun, Lijun[1];Wang, Yaling[1];Deng, Qi[1];Fang, Zhijia[1];Zhao, Liangzhong[2];Zhao, Jian[3]

机构：[1]Guangdong Ocean Univ, Guangdong Higher Educ Inst,Guangdong Prov Key Lab, Coll Food Sci & Technol,Guangdong Prov Engn Lab M, Key Lab Adv Proc Aquat Prod,Guangdong Prov Engn T, Zhanjiang 524088, Peoples R China;[2]Shaoyang Univ, Coll Food Sci & Chem Engn, Shaoyang 422000, Peoples R China;[3]Univ New South Wales, Sch Chem Engn, Sydney, NSW 2052, Australia

年份：2021

卷号：532

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:000600429200003)、、WOS

基金：This study was supported by the National Natural Science Foundation of China (NSFC) (No. 31871898) and Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams (No. 2019KJ149); and program for scientific research start-up fund of Guangdong Ocean University (R19050).

语种：英文

外文关键词：Penaeus vannamei; AFB(1) toxin; Tea polyphenols; Shrimp quality; Protein

外文摘要：Tea polyphenols (TP), as common antioxidants, have been widely used to mitigate for the protection and preservation of aquatic animals, but the protective effect of TP against the deterioration of shrimp muscle quality induced by aflatoxin B-1 (AFB(1)) has not been reported. This study was designed to analyse the root cause of AFB(1) induced declines in shrimp muscle quality and the protective mechanisms of TP at the molecular level. Shrimp were exposed to AFB(1) and TP for 20 d following a dose-escalating trial, and changes to the muscle histopathology, basic nutrient composition, and protein composition were determined. Non-labelled quantitative proteomics was used to screen and identify differential marker proteins associated with AFB(1) and TP. After 20 d of AFB(1) exposure, the water content, crude protein content and crude fat content of the shrimp muscle decreased to 68.66%, 18.27% and 1.01%, respectively, while the ash content was positively correlated with AFB(1) dose (R2 = 0.955). AFB(1) caused damage to the shrimp muscle microstructure in a dose-dependent manner, such as significant enlargement of muscle fibre spaces, rupture of the sarcomere, inflammation and so on. AFB1 induced changes in muscle protein composition, resulting in a decrease in the content of myofibrillar, sarcoplasmic and stroma proteins, while the alkali-soluble protein content increased significantly. TP can effectively protect against the damage to shrimp muscle caused by AFB(1) by inhibiting the expansion of muscle fibre spaces and inflammation. TP (0.04-0.16% feed) also had a significant protective effect against the decrease of muscle nutrients and changes in protein composition of shrimp caused by exposure to AFB1 (1.2-2.7 mg/kg feed) in the early and middle period (4-12 d), but the protective effect was weak or not noticeable in the late period (16-20 d). Eleven proteins were identified and screened as biomarker proteins of AFB(1) induced quality decline and TP protection, which were involved in energy metabolism (fructose 1,6-bisphosphatase; transaldolase; fructosebisphosphate aldolase; arginine kinase), redox reaction (hydroxyphenylpyruvate reductase; prostaglandin reductase 1; protein arginine N-methyltransferase 8), anti-inflammatory responses (5 '-nucleotidase; actindepolymerizing factor) and protein translation/folding (eukaryotic translation initiation factor 3; peptidylprolyl cis-trans isomerase). This study provides a better understanding of AFB(1) induced damage to shrimp quality and technical guidance for the use of TP to prevent the damage during the culture and processing of Penaeus vannamei.