文献类型：期刊文献

中文题名：聚乙二醇1000维生素E琥珀酸酯-皂皮皂素微乳液的制备, 表征及活性

英文题名：Preparation, Characterization and Activity of D-α-Tocopheryl Polyethylene Glycol 1000 Succinate-Saponin Microemulsion

作者：Lü, Qiyan[1]; Lin, Shiye[1]; Mi, Yani[1]; Qian, Yuewei[1]; Teng, Hui[1]; Chen, Lei[2]

机构：[1] College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; [2] College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524025, China

年份：2021

卷号：42

期号：24

起止页码：9

外文期刊名：Shipin Kexue/Food Science

收录：EI(收录号：20220211455854)

语种：中文

外文关键词：Particle size - Cytotoxicity - Drops - Glycoproteins - Polyethylene glycols - Polyethylenes - Zeta potential - Microemulsions - Cell proliferation - Emulsification - High resolution transmission electron microscopy - Particle size analysis

外文摘要：D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS), an inhibitor of P-glycoprotein (P-gp) with low toxicity and irritation, and the natural phytochemical quillaja saponin (QS), with a strong emulsifying effect, were selected for mixing with ethoxylated hydrogenated castor oil (RH40) as emulsifiers to prepare TPGS-QS microemulsion with ethyl oleate as an oil phase and isopropanol as a co-emulsifier.The morphology, particle size and zeta potential of emulsion droplets were observed by optical microscopy (OM), transmission electron microscopy (TEM) and laser particle size analyzer.The optimal microemulsion formulation consisted of a mixture of ethyl oleate, 0.02% TPGS, 1.5% QS, RH40 and isopropanol at a ratio of 30:6.6:6.6:39.4:17.5 (m/m).All solutions were prepared with purified water.The microemulsion was yellowish, clear and transparent in appearance with strong fluidity, and it was identified as an O/W-type emulsion with uniform spherical droplets.In addition, its average particle size was (48.89 ± 0.08) nm, and its zeta potential was(-4.513 ± 0.564) mV.The α-glycosidase inhibitory activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) cation radical scavenging capacity and iron reducing power of TPGS-QS microemulsion was tested, and its in vitro cytotoxicity on HepG2 and Caco-2 cells was evaluated through MTT assay.The half-maximal inhibitory concentration against α-glycosidase was 67.15 mg/mL, and the emulsionhad antioxidant activity.It hardly affected the normal growth of cells, but even had a promoting effect on cell proliferation within a certain concentration range. ? 2021, China Food Publishing Company. All right reserved.