文献类型：期刊文献

英文题名：Regulation of Thermostable Direct Hemolysin and Biofilm Formation of Vibrio parahaemolyticus by Quorum-Sensing Genes luxM and luxS

作者：Guo, Muhan[1];Fang, Zhijia[1];Sun, Lijun[1];Sun, Dongfang[1];Wang, Yaling[1];Li, Can[1];Wang, Rundong[1];Liu, Yang[2];Hu, Hanqiao[1];Liu, Ying[1];Xu, Defeng[1];Gooneratne, Ravi[3]

机构：[1]Guangdong Ocean Univ, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Guangdong Prov Engn Technol Res Ctr Marine Food, Coll Food Sci & Technol,Guangdong Prov Key Lab Aq, Zhanjiang 524088, Peoples R China;[2]Natl Marine Prod Qual Supervis & Inspect Ctr, Zhanjiang 524000, Peoples R China;[3]Lincoln Univ, Dept Wine Food & Mol Biosci, Canterbury 7647, New Zealand

年份：2018

卷号：75

期号：9

起止页码：1190

外文期刊名：CURRENT MICROBIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000440407200010)、、Scopus(收录号：2-s2.0-85047177640)、WOS

基金：This work was sponsored by grants from the Natural Science Foundation of China (No. 31371746) and the higher educational cultivation program for major scientific research projects of Guangdong Ocean University (Nos. GDOU2013050205, 2014050203) and the scientific research program of administration of quality and technology supervision of Guangdong province (No. 2015ZZ02).

语种：英文

外文摘要：Vibrio parahaemolyticus is a seafood opportunistic pathogen. There are evidences suggesting that virulence skills, including hemolytic activity and biofilm formation, are regulated by the luxM/luxS-dependent quorum-sensing system in V. parahaemolyticus, and their regulatory mechanism is not well understood. To better understand the virulence regulatory mechanism of V. parahaemolyticus, the luxM deletion (Delta luxM) and luxS deletion (Delta luxS) mutants were constructed and their impacts on growth, hemolysin activity, and biofilm were investigated. Results show that both luxM and luxS are involved in the adaptation to environmental conditions in early adaptive-log phase growth of V. parahaemolyticus. Thermostable direct hemolysin gene (tdh) was negatively regulated by luxM and positively regulated by luxS. The biofilm formation was negatively regulated by both luxS and luxM. This study provides an insight into some aspects of V. parahaemolyticus virulence regulation by luxM/luxS-dependent quorum-sensing system.