文献类型：期刊文献

中文题名：CⅢ-1菌株胞外抗菌蛋白防治香蕉炭疽病研究

英文题名：Biological control to anthracnoses in banana by antimicrobial proteins isolation from strain CⅢ-1

作者：柳凤[1,2];陈振明[1];何红[1]

机构：[1]广东海洋大学农学院;[2]中国热带农业科学院南亚热带作物研究所

年份：2010

卷号：27

期号：4

起止页码：580

中文期刊名：果树学报

外文期刊名：Journal of Fruit Science

收录：CSTPCD、、北大核心2008、CSCD_E2011_2012、北大核心、CSCD

基金：广东省科技计划(2009B020310015;2009B030803055)

语种：中文

中文关键词：香蕉;抗菌蛋白;炭疽病;生物防治;机制

外文关键词：Bananas; Antimicrobial proteins; Anthracnose; Bio-control; Mechanism

中文摘要：为明确红树内生细菌CⅢ-1菌株的抗菌蛋白对香蕉炭疽病的控制作用,采用平板对峙法结合显微观察测定其对致病菌的抑制活性,进行抗菌蛋白对香蕉炭疽病的防病试验,并测定了香蕉果实内丙二醛的含量及防御酶活性的变化。结果表明,CⅢ-1抗菌蛋白粗提液喷雾后立即接种香蕉炭疽病菌,处理8d后其防治效果达71.44%,10d后防效仍有56.67%;防病机制测定表明,(1)CⅢ-1抗菌蛋白对香蕉炭疽病菌的菌丝生长及分生孢子萌发等具有极强的抑制作用,处理后的病菌菌丝出现肿胀、膨大、成泡囊状畸形,分生孢子基本不萌发。(2)抗菌蛋白处理香蕉后,与对照比,抗菌蛋白处理的香蕉丙二醛(MDA)的含量较低,同时过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)等活性也均有不同程度的变化。

外文摘要：In order to evaluate the efficacy of the extra cellular antimicrobial proteins （EACP） of CⅢ-1 to anthracnoses in banana, the inhibition effect on the pathogen of Colletotrichum musae were tested using dual culture method and optical microscope observation, plant biocontrol tests were also carried out with EACP and the change in the content of MDA and activity of SOD, POD and PAL were measured as well. The results showed that： the control effects of the ECAP on the banana anthracnoses were 71.44% and 56.67% 8 d and 10 d after application,respectively. This suggested that the anthracnose of banana was controlled effectively with the ECAP. The research on biological control mechanisms showed that ECAP could inhibit the mycelial growth and reduce the germination of Colletotrichum musae. And treatment with ECAP could reduce the contents of MDA, and at same time could induce the variances of the activities of POD and PAL in the banana and capsicum fruits, cormpared with sterile water and PSB treatment.