文献类型：期刊文献

英文题名：Vitellogenin Receptor (VgR) Mediates Oocyte Maturation and Ovarian Development in the Pacific White Shrimp (Litopenaeus vannamei)

作者：Ruan, Yao[1,2];Wong, Nai-Kei[3];Zhang, Xin[1,2];Zhu, Chunhua[4];Wu, Xiaofen[1,2];Ren, Chunhua[1,5];Luo, Peng[1,5];Jiang, Xiao[1,5];Ji, Jiatai[1,6];Wu, Xugan[7];Hu, Chaoqun[1,5];Chen, Ting[1,5]

机构：[1]Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, South China Sea Inst Oceanol, Guangzhou, Peoples R China;[2]Univ Chinese Acad Sci, Beijing, Peoples R China;[3]Southern Univ Sci & Technol, Hosp 2, Natl Clin Res Ctr Infect Dis, Shenzhen Peoples Hosp 3, Shenzhen, Peoples R China;[4]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Peoples R China;[5]Chinese Acad Sci, Inst South China Sea Ecol & Environm Engn ISEE, Guangzhou, Peoples R China;[6]Guangdong Haimao Investment Co Ltd, Zhanjiang, Peoples R China;[7]Shanghai Ocean Univ, Key Lab Freshwater Aquat Genet Resources, Minist Agr, Shanghai, Peoples R China

年份：2020

卷号：11

外文期刊名：FRONTIERS IN PHYSIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000538425300001)、、Scopus(收录号：2-s2.0-85085592077)、WOS

基金：This study was supported by Science & Technology Project of Guangzhou (201904020009), Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (2019KJ149), Guangdong Province Program (2017B030314052, 2017A030310429, and 2016A020210062), Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences (ISEE2018PY03), and Shanghai talents development fund for young scientists (No. 2018100).

语种：英文

外文关键词：vitellogenin receptor; crustacean; phylogenetic analysis; vitellogenesis; mRNA expression; ovarian development; oocyte maturation

外文摘要：Oocyte maturation and ovarian development are sequentially coordinated events critical to reproduction. In the ovaries of adult oviparous animals such as birds, bony fish, insects, and crustaceans, vitellogenin receptor (VgR) is a plasma membrane receptor that specifically mediates vitellogenin (Vg) transport into oocytes. Accumulation of Vg drives sexual maturation of the female crustaceans by acting as a pivotal regulator of nutritional accumulation within oocytes, a process known as vitellogenesis. However, the mechanisms by which VgR mediates vitellogenesis are still not fully understood. In this study, we first identified a unique VgR (Lv-VgR) and characterized its genomic organization and protein structural domains in Litopenaeus vannamei, a predominant cultured shrimp species worldwide. This newly identified Lv-VgR phylogenetically forms a group with VgRs from other crustacean species within the arthropod cluster. Duplicated LBD/EGFD regions are found exclusively among arthropod VgRs but not in paralogs from vertebrates and nematodes. In terms of expression patterns, Lv-VgR transcripts are specifically expressed in ovaries of female shrimps, which increases progressively during ovarian development, and rapidly declines toward embryonic development. The cellular and subcellular locations were For analyzed by in situ hybridization and immunofluorescence, respectively. The Lv-VgR mRNA was found to be expressed in the oocytes of ovaries, and Lv-VgR protein was found to localize in the cell membrane of maturing oocytes while accumulation of the ligand Vg protein assumed an even cytoplasmic distribution. Silencing of VgR transcript expression by RNAi was effective for stunting ovarian development. This present study has thus provided new insights into the regulatory roles of VgR in crustacean ovarian development.