文献类型：期刊文献

英文题名：Neurokinin B signaling in hermaphroditic species, a study of the orange-spotted grouper (Epinephelus coioides)

作者：Chen, Huapu[1,2];Xiao, Ling[3,4];Liu, Yali[3,4];Li, Shuisheng[3,4];Li, Guangli[1];Zhang, Yong[3,4];Lin, Haoran[3,4]

机构：[1]Guangdong Ocean Univ, Zhanjiang City Key Lab Marine Ecol & Environm, Guangdong Res Ctr Reprod Control & Breeding Techn, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China;[3]Sun Yat Sen Univ, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China;[4]Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China

年份：2018

卷号：260

起止页码：125

外文期刊名：GENERAL AND COMPARATIVE ENDOCRINOLOGY

收录：SCI-EXPANDED(收录号：WOS:000428360500015)、、WOS

基金：This study was supported by grants from the National Natural Science Foundation of China (Nos. 31672631, 31572596, 41706174 and 31702326), the Special Fund for the Development of Marine and Fisheries (Nos. A201608B01 and 2017A0012), The China Agriculture Research System (CARS-47), the Guangdong Provincial Natural Science Foundation (2015A030313069) and Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology (No. 2016LMFS-B12).

语种：英文

外文关键词：NKB; Gene expression; Reproductive axis

外文摘要：Neurokinin B (NKB) plays important roles in the mammalian reproductive axis by modulating the release of gonadotropin-releasing hormone (GnRH) and gonadotropins. In the present study, the tac3 cDNA was cloned from a hermaphroditic species, the orange-spotted grouper. Sequence analysis showed that the grouper Tac3 precursor encoded two tachykinin peptides, NKB and NKB-related peptide (NKBRP). Expression analysis in different tissues revealed that tac3 mRNA was highly expressed in the brain of the orange-spotted grouper. In situ hybridization further revealed that it was localized in some hypothalamic nuclei associated with reproductive regulation. During ovarian development, an increase of tac3 expression in the hypothalamus was observed at vitellogenesis stage. Intraperitoneal administration of NKB could increase the gnrhl and lh beta mRNA levels, and enhance the serum estrogen levels, but did not significantly influence 110 expression in cultured pituitary cells, indicating that NKB does not directly exert its actions on the pituitary gland. However, it was found that NKBRP had no effect on the expression of two gnrhs and two gths in vivo and in vitro. Effects of sex steroids on tac3 expression were further investigated. During the 17-methyltestosterone-induced sex change in the orange-spotted grouper, hypothalamic tac3 expression showed no significant change. Interestingly, ovariectomy greatly stimulated tac3 expression, while the 17 beta-estradiol treatment reversed this effect. In general, our data highly indicated that NKB signaling could activate the reproductive axis in the orange-spotted grouper. Our study is the first description of the NKB signaling in the hermaphroditic species. (C) 2018 Elsevier Inc. All rights reserved.