文献类型：期刊文献

英文题名：Determination of multiple phytohormones in fruits by high-performance liquid chromatography with fluorescence detection using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling

作者：Li, Guoliang[1,2];Lu, Shuaimin[1];Wu, Hongliang[3];Chen, Guang[1];Liu, Shucheng[2];Kong, Xiaojian[4];Kong, Weiheng[1];You, Jinmao[1,3]

机构：[1]Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Shandong, Peoples R China;[2]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China;[3]Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China;[4]Shan Dong Career Dev Coll, Jining, Peoples R China

年份：2015

卷号：38

期号：2

起止页码：187

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000348516600003)、、EI(收录号：20150100393445)、Scopus(收录号：2-s2.0-84920053864)、WOS

基金：This work was supported by The National Natural Science Foundation of China (Nos. 31301595, 21475074, and 21275089), Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety (GDPKLAPPS1401), the Natural Science Foundation of Shandong Province, China (ZR2013BQ019), PhD research start-up funds of Qufu Normal University (bsqd 2012017), and the Soft Science Project of Shandong Province (2013RKA08020).

语种：英文

外文关键词：Fluorescent labeling; Fruits; High-performance liquid chromatography; Plant hormones

外文摘要：Plant hormone determination in food matrices has attracted more and more attention because of their potential risks to human health. However, analytical methods for the analysis of multiple plant hormones remain poorly investigated. In the present study, a convenient, selective, and ultrasensitive high-performance liquid chromatography method for the simultaneous determination of multiple classes of plant hormones has been developed successfully using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling. Eight plant hormones in fruits including jasmonic acid, 12-oxo-phytodienoic acid, indole-3-acetic acid, 3-indolybutyric acid, 3-indolepropionic acid, gibberellin A(3), 1-naphthylacetic acid, and 2-naphthaleneacetic acid were analyzed by this method. The conditions employed for dispersive liquid-liquid microextraction were optimized systematically. The linearity for all plant hormones was found to be >0.9993 (R-2 values). This method offered low detection limits of 0.19-0.44 ng/mL (at a signal-to-noise ratio of 3), and method accuracies were in the range of 92.32-103.10%. The proposed method was applied to determine plant hormones in five kinds of food samples, and this method can achieve a short analysis time, low threshold levels of detection, and a high specificity for the analysis of targeted plant hormones present at trace level concentrations in complex matrices.