文献类型：期刊文献

中文题名：月桂酸单甘油酯对凡纳滨对虾生长、肌肉氨基酸、非特异性免疫及肠道菌群的影响

英文题名：Effects of glycerol monolaurate on growth performance,muscle amino acids,non-specific immunity and intestinal flora of Litopenaeus vannamei

作者：韦宏杰[1,2];李雪鹤[1,2];吴远彩[1,2];易远名[1,2];朱东文君[1,2];杨奇慧[1,2,3];谭北平[1,2,3]

机构：[1]广东海洋大学水产学院,水产经济动物营养与饲料实验室,广东湛江524088;[2]广东省水产动物精准营养与高效饲料工程技术研究中心,广东湛江524088;[3]广东省水产动物病害防控与健康养殖重点实验室,广东湛江524088

年份：2022

卷号：46

期号：10

起止页码：1912

中文期刊名：水产学报

外文期刊名：Journal of Fisheries of China

收录：CSTPCD、、CSCD2021_2022、Scopus、北大核心、CSCD、北大核心2020

基金：国家自然科学基金(31802316);国家重点研发计划(2019YFD0900200);广东省科技特派员项目(GDKTP2021048400)。

语种：中文

中文关键词：凡纳滨对虾;月桂酸单甘油酯;生长;非特异性免疫;抗病性;肠道菌群

外文关键词：Litopenaeus vannamei;glycerol monolaurate;growth;non-specific immunity;disease resistance;intestinal flora

中文摘要：为研究饲料中添加月桂酸单甘油酯(GML)对凡纳滨对虾幼体生长、免疫、肌肉氨基酸及肠道菌群的影响。实验设计分别含有0、500、1000、1500、2000和2500 mg/kg GML的6组等氮等脂实验饲料,对凡纳滨对虾幼体[体质量为(0.31±0.02)g]进行为期56 d的养殖实验。结果显示,①与对照组相比,添加2000 mg/kg GML能显著提高增重率和特定生长率并显著降低饲料系数。②饲料中添加GML对凡纳滨对虾体成分影响不显著。③各添加组凡纳滨对虾尾肌呈味氨基酸及总氨基酸含量显著高于对照组。④与对照组相比,各添加水平的血清总蛋白含量均显著上升,低密度蛋白胆固醇含量及谷草转氨酶活性均显著下降;500和1500 mg/kg组甘油三酯含量显著下降。⑤当GML添加水平为1500 mg/kg时,超氧化物歧化酶活性和过氧化氢酶表达量显著上调,GML添加水平超过2000 mg/kg时,IMD、Toll表达量以及溶菌酶活性显著上调,2000 mg/kg组酚氧化酶原表达量显著上调。⑥添加组Chao1指数和Ace指数显著上调,1500 mg/kg组和2500 mg/kg组Shannon指数显著下调,2500 mg/kg组Simpson指数显著下调。实验表明,添加适宜水平的GML可提高凡纳滨对虾幼体的生长性能、非特异性免疫效应,且GML可下调肠道有害菌的丰度,改善肠道菌群结构。以增重率为衡量指标,饲料中添加2142.99 mg/kg GML对凡纳滨对虾的生长效果最佳。

外文摘要：A feed trial was conducted in Litopenaeus vannamei to evaluate the effects of glycerol monolaurate(GML)supplement on growth,muscle amino acids,non-specific immunity,disease resistance,and intestinal flora structure.Juvenile L.vannamei[body weight=(0.31±0.02)g]were fed for 56 days with six iso-nitrogenous and isoenergetic experimental diets containing 0(control group),500,1000,1500,2000 and 2500 mg/kg GML,respectively.The results showed that,①2000 mg/kg GML significantly increased the weight gain rate and specific growth rate and significantly reduced the feed coefficient compared to the control group.②Feeding L.vannamei with GML diet had no significant effect on the body composition of the fish.③L.vannamei fed with GML diet had significantly higher content of flavor amino acids and total amino acids.④Serum total protein content was significantly higher,LDL cholesterol content and glutathione transaminase activity were significantly lower in all addition groups;triglyceride content was significantly lower in those fed with 500 mg/kg and 1500 mg/kg GML.⑤Superoxide dismutase activity and catalase expression were significantly up-regulated in those fed with 1500 mg/kg GML,and IMD,Toll expressions,and lysozyme activity were significantly up-regulated in those fed with 2000 and 2500 mg/kg GML,and phenol oxidase pro expression was significantly up-regulated in those fed with 2000 mg/kg GML.⑥All addition groups had significantly increased Chao1 and Ace indexes,L.vannamei fed with 1500 and 2500 mg/kg GML had significantly decreased Shannon index,and Simpson index was significantly decreased in those fed with 2500 mg/kg.In summary,the results of the present study emphasized the favorable effects of adequate dietary GML on growth performance and non-specific immunity and intestinal flora.Dietary GML at 2142.99 mg/kg GML was recommended to maintain optimal growth performance of L.vannamei.