文献类型：期刊文献

英文题名：Silencing of Nrf2 in Litopenaeus vannamei, decreased the antioxidant capacity, and increased apoptosis and autophagy

作者：Huang, Yongxiong[1,2];Li, Qi[1];Yuan, Yunhao[1];Zhang, Zhiqiang[1];Jiang, Baijian[1];Yang, Shiping[1,2];Jian, Jichang[1,2]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Southern Marine Sci & Engn Guangdong Lab,Key Lab, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[2]Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen, Peoples R China

年份：2022

卷号：122

起止页码：257

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000770641400006)、、WOS

基金：Acknowledgment This work was supported by the National Natural Science Foundation of China (No. U20A2065, 32073006, 32002426) , National Key R&D Program of China (No. 2018YFD0900501) , Fund of Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) (No. ZJW-2019-06) , Project of the agricultural sci-tech commissioners of Guang-dong province (KTP20210292) .

语种：英文

外文关键词：Nrf2; RNA interference; Litopenaeus vannamei; Antioxidant capacity; Apoptosis; Autophagy

外文摘要：Nuclear factor E2-related factor 2 (Nrf2) is a multifunctional transcription factor that plays an important role in antioxidant activities. However, its effect on antioxidant capacity in Litopenaeus vannamei, an economically important crustacean, remains unclear. In this study, the role of Nrf2 in response to oxidative stress in L. vannamei was determined by its effect on relevant gene expression and enzymatic activity. Nrf2 was cloned and analyzed. Results revealed that Nrf2 contains a 1575 bp open reading frame encoding 524 amino acids and a conserved bZIP Maf domain. The sequence similarity of Nrf2 between L. vannamei and Homarus americanus is 81%. Although the Nrf2 expression was detected in all tissues, the Nrf2 expression levels were the highest in the hepatopancreas, followed by the eyestalk and muscle. RNA interference significantly decreased the expression of antioxidant-related genes (SOD, GPX, CAT, Trx, and HO-1; p < 0.05), significantly upregulated the expression of autophagy genes (Atg3, Atg4, Atg5, Atg10, and Atg12; p < 0.05) and apoptosis genes (Caspase-3 and P53; p < 0.05). Moreover, SOD, CAT, and GPX enzyme activities decreased whereas the MDA activity increased. The histological results of the shrimp injected with dsRNA-Nrf2 showed that the hepatic tubules were irregularly arranged, the lumen was abnormal, and a few hepatic tubules were significantly enlarged compared with those of the dsRNA-EGFP group. The hepatocytes were also vacuolated. In conclusion, this study provided evidence that Nrf2 is involved in the regulation of antioxidant capacity, oxidative stress, apoptosis, and autophagy in shrimp.