文献类型：期刊文献

英文题名：Catechol functionalized chitosan/active peptide microsphere hydrogel for skin wound healing

作者：Zhang, Dongying[1,2];Ouyang, Qianqian[3];Hu, Zhang[1];Lu, Sitong[1];Quan, Weiyan[1];Li, Puwang[4];Chen, Yu[5];Li, Sidong[1]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhanjian, Zhanjiang 524000, Peoples R China;[3]Guangdong Med Univ, Marine Biomed Res Inst, Guangdong Key Lab Res & Dev Nat Drugs, Zhanjiang 524023, Peoples R China;[4]Chinese Acad Trop Agr Sci, South Subtrop Crop Res Inst, Zhanjiang 524001, Peoples R China;[5]Beijing Inst Technol, Sch Mat Sci & Engn, Beijing 100081, Peoples R China

年份：2021

卷号：173

起止页码：591

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:000623235400053)、、EI(收录号：20240815593633)、Scopus(收录号：2-s2.0-85099930377)、WOS

基金：We gratefully acknowledge the assistance of Prof. L.X. Kong in revising the manuscript, and the financial support by Guangdong Provincial Natural Science Foundation of China (2016A030308009 and 2018A030307015), Science and Technology Planning Project of Guangdong Province (2015A020216019), Project of Science and Technology Plan of Zhanjiang (2019A01017), Project of Enhancing School with Innovation of Guangdong Ocean University (2017KTSCX090) and Postgraduate Education Innovation Program of Guangdong Ocean University (201926 and 201927).

语种：英文

外文关键词：Chitosan; Active oyster peptide; Hydrogel; Wound healing

外文摘要：Chitosan-based thermosensitive hydrogels have been widely used in drug delivery and tissue engineering, but their poor bioactivity has limited their further applications. Integral active oyster peptide microspheres (OPM) with an average particle diameter of 3.9 mu m were prepared with high encapsulation efficiency (72.8%) and loading capacity (11.9%), exhibiting desirable sustained release effects. Using catechol functionalized chitosan (CS-C) as the polymeric matrix, OPM as the filler, and beta-sodium glycerophosphate (beta-GP) as a thermal sensitizer, the thermosensitive hydrogel CS-C/OPM/beta-GP was prepared. Besides, the application of the hydrogel on wound healing was studied, and its biosafety was evaluated. The results of cell migration in vitro showed that the cell migration rate of CS-C/OPM/beta-GP reached 97.47 +/- 5.41% within 48 h, indicating that the hydrogel accelerated the migration of L929 cells. As demonstrated in the mouse skin wound experiment, CS-C/OPM/beta-GP hydrogel not only inhibited the aggregation of diversified inflammatory cells and accelerated the generation of collagen fibers and new blood vessels of the wound, but also enhanced the synthesis of total protein (TP) in granulation tissue, and up-regulated the expression of Ki-67 and VEGF in the injury, thereby achieving fast wound healing. Safety evaluation results showed that CS-C/OPM/beta-GP hydrogel was not cytotoxic to L929 cells, and the hemolysis ratio was less than 5% within 1 mg/mL. In conclusion, CS-C/OPM/beta-GP hydrogel is expected as a promising medical dressing for wound healing. (C) 2021 Published by Elsevier B.V.