文献类型：期刊文献

英文题名：Dietary replacement of fish meal with peanut meal in juvenile hybrid grouper (Epinephelus fuscoguttatus female x Epinephelus lanceolatus male): Growth performance, immune response and intestinal microbiota

作者：Ye, Guanlin[1];Dong, Xiaohui[1,2,3];Yang, Qihui[1,2,3];Chi, Shuyan[1,2,3];Liu, Hongyu[1,2,3];Zhang, Haitao[2,4];Tan, Beiping[1,2,3];Zhang, Shuang[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Tech, Zhanjiang 524088, Peoples R China;[3]Aquat Anim Precis Nutr & High Efficiency Feed Eng, Zhanjiang 524088, Peoples R China;[4]Res Ctr Guangdong Evergreen Feed Ind Co Ltd, Zhanjiang 524022, Peoples R China

年份：2020

卷号：17

外文期刊名：AQUACULTURE REPORTS

收录：SCI-EXPANDED(收录号：WOS:000544613100006)、、WOS

基金：This study was financially supported by the National Key R&D Program of China (2019YFD0900200), the China Agriculture Research System (CARS-47) and the National Natural Science Foundation of China (NSFC 31772864).

语种：英文

外文关键词：Epinephelus fuscoguttatus female x E. lanceolatus male; Peanut meal; Growth performance; Intestinal microbiota; Immunity

外文摘要：The objective of the present study was to assess the effects of substituting fishmeal (FM) with peanut meal (PNM) on growth performance, immunity, and intestinal microbiota in juvenile hybrid grouper (Epinephelus fusco- guttatus female x Epinephelus lanceolatus male). Hybrid grouper (initial weight 16.85 +/- 0.02 g) were fed diets (iso- nitrogenous and isolipidic) with PNM replacement rates of 0% (FM), 10 % (PNM10), 20 % (PNM20), 30 % (PNM30), 40 % (PNM40) and 50 % (PNM50) FM protein, respectively. The growth trial lasted for a 10-week period. The results showed that FM replaced by PNM had no significant effect on the growth performance, feed utilisation, somatic indices, and whole-body composition of hybrid grouper (P 0.05), but with replacement rates of up to 50 %, there was no significant difference from the FM group (P 0.05). The mRNA expression of epinecidin and hepcidin in the PNM40 and PNM50 groups was significantly lower than in the FM group (P < 0.05). The gut bacterial community of the distal intestine was analysed by Next-Generation Sequencing. At the phylum level, the relative abundance of Bacteroidetes increased first and then reduced with the increasing rate of re- placement, reaching the maximum in the PNM20 group. At the family level, the relative abundance of Bacteroidales S24-7 group had the same trend. The relative abundance of Vibrionaceae grew with the increased replacement level, reaching the maximum in the PNM50 group. Functional predictions of the distal intestinal microbiota indicated that the folding, sorting, and degradation pathway was significantly diminished with the increased replacement level (P < 0.05). The glycan biosynthesis and metabolism, and the immune system pathway of the PNM50 group were significantly lower than those of the FM group, while the metabolism of terpenoids and polyketides showed an opposite trend (P < 0.05). In conclusion, replacing FM with PNM of up to 50 % had no significant negative influence on growth performance, but obviously changed the immunity and intestinal microbiota of juvenile hybrid grouper (E. fuscoguttatus female x E. lanceolatus male).