文献类型：期刊文献

中文题名：珠母贝糖胺聚糖PG-Ⅱ诱导人宫颈癌HeLa细胞凋亡的研究

英文题名：HeLa Cells Apoptosis Induced by Glycosaminoglycan Extracted from Pinctada martensi Dunker

作者：吴红棉[1];金晓石[1];范秀萍[1];胡雪琼[1];钟敏[1];雷晓凌[1]

机构：[1]广东海洋大学食品科技学院,广东湛江524088

年份：2012

卷号：33

期号：3

起止页码：238

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD、、北大核心2011、Scopus、CSCD2011_2012、北大核心、CSCD

基金：广东省教育厅自然科学研究项目(04J006)

语种：中文

中文关键词：珠母贝糖胺聚糖;人宫颈癌HeLa细胞;细胞凋亡

外文关键词：glycosaminoglycan from Pinctada martensi Dunker; HeLa cell; apoptosis

中文摘要：目的:探讨珠母贝糖胺聚糖PG-II诱导人宫颈癌HeLa细胞凋亡,从而抑制其增殖。方法:MTT法检测珠母贝糖胺聚糖PG-II对人宫颈癌HeLa细胞生长的抑制作用。倒置显微镜、荧光显微镜观察HeLa细胞凋亡的形态学特征,琼脂糖凝胶电泳检测DNA断裂片段,流式细胞仪定量检测细胞凋亡的百分率及对其细胞周期的影响。结果:MTT法显示PG-II可抑制人宫颈癌HeLa细胞的增殖。倒置显微镜和荧光显微镜形态学观察可见HeLa细胞凋亡的形态明显变化。琼脂糖凝胶电泳显示PG-II作用HeLa细胞24h,出现分子质量为200bp到2000bp不等的细胞凋亡的特征性DNA梯状条带。流式细胞术测试表明:PG-II可使HeLa细胞阻滞于G1期,剂量为100mg/L作用24h阻滞作用最明显,凋亡指数为42.6%。结论:珠母贝糖胺聚糖PG-II可能通过诱导人宫颈癌HeLa细胞凋亡而抑制其增殖。

外文摘要：Objective： To investigate the apoptosis-inducing and proliferation-inhibitory effect glycosaminoglycan PG-II extracted from Pinctada martensi Dunker on HeLa cells. Methods： The growth-inhibitory effect was tested by MTT assay. Morphological observations on HeLa cells apoptosis were carried out under inverted microscope and inverted fluorescence microscope. The biochemical changes of HeLa cell DNA during apoptosis were observed by agarose gel electrophoresis. The percentage of apoptotic cells and cell cycle blockage were detected by flow cytometry. Results： PG- II could inhibit the proliferation of HeLa cells as determined by MTr assay. Morphological changes in apoptotic HeLa cells were observed under inverted microscope and inverted fluorescence microscope. The agarose gel electropherogram of HeLa cells treated with PG-II for 24 h displayed characteristic DNA ladders from apoptotic HeLa cells with molecular mass between 200 bp and 2000 bp. The results of flow cytometry indicated that PG- H could block the HeLa cell cycle at the G1 phase and that the largest blocking effect was achieved after treatment for 24 h at the dose of 100 mg/L with an apoptosis index of 42.6%. Conclusion： PG- II can induce apoptosis in HeLa cells and consequently inhibit their proliferation.