文献类型：期刊文献

英文题名：Two Novel Bioactive Peptides from Antarctic Krill with Dual Angiotensin Converting Enzyme and Dipeptidyl Peptidase IV Inhibitory Activities

作者：Ji, Wei[1];Zhang, Chaohua[1,2,3,4];Ji, Hongwu[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang, Peoples R China;[2]Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China;[3]Guangdong Higher Educ Inst, Key Lab Adv Proc Aquat Prod, Zhanjiang, Peoples R China;[4]South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China

年份：2017

卷号：82

期号：7

起止页码：1742

外文期刊名：JOURNAL OF FOOD SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000405525900032)、、Scopus(收录号：2-s2.0-85019937078)、WOS

基金：This research program was co-supported by the Modern Agroindustry Technology Research System of China (No. CARS-47); Disciplinary Construction Project of Institutions of Higher Learning in Guangdong Province (No. 2013CXZDA020); Guangdong Ocean Univ. Strong School Innovation Fund Project (No. Q14202) and Industry-University Research Cooperation Project in Guangdong Province (No. 2013B090600155).

语种：英文

外文关键词：ACE; dual inhibitory peptides; DPP-IV; purification; TOF-MS/MS

外文摘要：Inhibition of dipeptidyl peptidase IV (DPP-IV) and angiotensin converting enzyme (ACE) are considered useful in managing 2 often associated conditions: diabetes and hypertension. In this study, corolase PP was used to hydrolyze Antarctic krill protein. The hydrolysate (AKH) was isolated by ultrafiltration and purified by size-exclusion chromatography, ion exchange chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC) sequentially. The in vitro inhibitory activities of all AKHs and several fractions obtained against ACE and DPP-IV were assessed. Two peptides, purified with dual-strength inhibitory activity against ACE and DPP-IV, were identified by TOF-MS/MS. Results indicated that not all fractions exhibited dual inhibitory activities of ACE and DPP-IV. The purified peptide Lys-Val-Glu-Pro-Leu-Pro had half-maximal inhibitory concentrations (IC50) of 0.93 +/- 0.05 and 0.73 +/- 0.04 mg/mL against ACE and DPP-IV, respectively. The other peptide Pro-Ala-Leu had IC50 values of 0.64 +/- 0.05 and 0.88 +/- 0.03 mg/mL against ACE and DPP-IV, respectively. This study firstly reported the sequences of dual bioactive peptides from Antarctic krill proteins, further provided new insights into the bioactive peptides responsible for the ACE and DPP-IV inhibitory activities from the Antarctic krill protein hydrolysate to manage hypertension and diabetes.