文献类型：期刊文献

中文题名：尼罗罗非鱼清道夫受体基因Scara3的克隆及病原微生物刺激后的表达响应

英文题名：Cloning and expression response of Scara3 gene to pathogenic microorganism stimulation in Nile tilapia Oreochromis niloticus

作者：胡惠玲[1,2];汪志文[1,2];黎源[1,2];鲁义善[1,2];简纪常[1]

机构：[1]广东海洋大学水产学院,广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524088;[2]广东海洋大学深圳研究院,广东省水生动物健康评估工程技术研究中心,深圳市海水经济动物种苗健康评价公共技术平台,广东深圳518120

年份：2022

卷号：37

期号：1

起止页码：71

中文期刊名：大连海洋大学学报

外文期刊名：Journal of Dalian Ocean University

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：深圳市科技计划项目(JCYJ20180306173023022);深圳市大鹏新区科技创新和产业发展专项资金项目(PT202101-23)。

语种：中文

中文关键词：尼罗罗非鱼;清道夫受体3基因;无乳链球菌;聚肌胞苷酸

外文关键词：Oreochromis niloticus;Scara3;Streptococcus agalactiae;Poly I:C

中文摘要：为探究清道夫受体3(scavenger receptor class a member 3,Scara3)在尼罗罗非鱼Oreochromis niloticus抵御病原微生物过程中的作用,采用聚合酶链式反应克隆得到罗非鱼Scara3基因,利用荧光定量PCR技术分析Scara3在健康尼罗罗非鱼组织分布及细菌、病毒感染后的表达情况,并进行亚细胞定位分析。结果表明:克隆得到尼罗罗非鱼Scara3基因cDNA全长序列为3889 bp,开放阅读框(ORF)为1827 bp,编码608个氨基酸,具有跨膜结构域和胶原(Collagen)结构域;多序列比对发现,Scara3氨基酸序列在脊椎动物中相对保守;亚细胞定位显示,Scara3蛋白在全细胞分布;荧光定量PCR分析显示,Scara3基因在健康罗非鱼各组织中均有表达,在血液、肠道、皮肤和胸腺等组织中表达量较高且显著高于其他组织(P<0.05);经脂多糖(LPS)、灭活无乳链球菌Streptococcus agalactiae和聚肌胞苷酸(Poly I:C)刺激罗非鱼头肾白细胞后,Scara3表达量显著上调(P<0.05),经灭活无乳链球菌和Poly I:C刺激后,肠道、头肾和皮肤组织中的基因表达量表现出时序性。研究表明,尼罗罗非鱼在对细菌和病毒感染的免疫反应活动中,Scara3基因起着重要作用,本研究结果为后期硬骨鱼类中Scara3基因的功能研究提供了科学参考。

外文摘要：To investigate the role of scavenger receptor class a member 3(Scara3)in the resistant to pathogenic microorganism of Nile tilapia Oreochromis niloticus,the Scara3 gene was cloned by polymerase chain reaction(PCR).The expression of scara3 in healthy and infected by bacteria and viruses Nile tilapia tissues distribution was detected by real-time fluorescent quantitative PCR.Meanwhile,we studied the localization of Scara3 in HEK-293T cells.The results showed that Scara3 gene had full-length cDNA sequence of 3889 bp,including open reading frame(ORF)of 1827 bp,and encoding 608 amino acids,and had transmembrane region and collagen domain.Multiple sequence alignment indicated that Scara3 was conserved among vertebrates.Subcellular localization showed that Scara3 were evenly distributed in the whole cell.The real time quantitative PCR revealed that the expression of Scara3 was detected in all tissues of healthy Nile tilapia,relatively high expression levels in blood,intestine,skin tissues and significantly higher than other tissues(P<0.05).The significantly up-regulated expression levels of Scara3 gene were observed in the head kidney leukocytes stimulated by lipopolysaccharide(LPS),inactivated S.agalactiae and polyinosinic acid(Poly I:C)(P<0.05).Scara3 gene expression appeared temporally in intestine,head kidney and skin tissues.These findings indicate that Scara3 gene plays an important role in the host immune response of Nile tilapia to bacterial and viral infections,which provides a theoretical basis for future studies on the function of Scara3 gene in bony fish.