文献类型：期刊文献

中文题名：棉籽蛋白源对大口黑鲈生长、体组成及健康的影响

英文题名：Effects of cottonseed protein source on growth,body composition and health of Micropterus salmoides

作者：周兴梅[1];何光伦[1];许志城[1];陈拥军[1];谭北平[2];林仕梅[1]

机构：[1]西南大学水产学院,淡水鱼类资源与生殖发育教育部重点实验室,重庆400716;[2]广东海洋大学水产学院,广东湛江524088

年份：2021

卷号：45

期号：10

起止页码：1703

中文期刊名：水产学报

外文期刊名：Journal of Fisheries of China

收录：CSTPCD、、CSCD2021_2022、Scopus、北大核心、CSCD、北大核心2020

基金：蓝色粮仓科技创新(2019YFD0900200);重庆市技术创新与应用发展(面上项目)(cstc2020jscxmsxmX0046)。

语种：中文

中文关键词：大口黑鲈;棉籽蛋白;生长;肝功能;肠道健康

外文关键词：Micropterus salmoides;cottonseed protein;growth performance;liver function;intestinal health

中文摘要：为评价棉籽蛋白在大口黑鲈饲料中应用的可行性,用4种不同质量的棉籽蛋白替代30%鱼粉配制成5种等氮等脂的实验饲料(CPC_(3)为对照组,CPC_(3),CPC_(3),CPC_(3),CPC_(3)),在室内循环养殖系统饲喂大口黑鲈[平均体质量(12.20±0.11)g]8周。结果显示,4种棉籽蛋白的营养组成、棉酚、棉籽糖和水苏糖含量不同,以CPC_(3)棉籽蛋白质量最优。CPC_(3)组鱼的终末体质量、增重率和特定生长率显著高于其他组,而各实验组全鱼常规营养成分和肌肉氨基酸组成没有显著差异。CPC_(3)组肝脏SOD、GSH-Px活性以及CAT和SOD mRNA表达水平最高,MDA含量最低。CPC_(3)组肝脏抗炎因子IL-10、TGF-β的相对表达量最高,而促炎因子IL-1β、IL-8和TNF-α的相对表达量最低。此外,棉籽蛋白源会显著影响大口黑鲈肝脏蛋白质代谢,CPC_(3)组ALT、AST活性以及基因P13K、AKT、m-TOR、S6K1、4E-BP表达水平最高。同时发现,CPC_(3)组大口黑鲈肠道SOD酶活性最高、MDA含量最低,且肠道通透性指标(二胺氧化酶活性、D-乳酸和内毒素含量)也最低。棉籽蛋白源也影响了肠道紧密连接蛋白相关基因ZO-1、Claudin-1和Occludin的表达。研究表明,棉籽蛋白质量对大口黑鲈生长和健康会产生显著影响,其中棉籽蛋白CPC_(3)效果最佳,显著改善大口黑鲈的肝脏和肠道健康,进而促进其生长。因此,棉籽蛋白CPC_(3)可以作为大口黑鲈饲料的优质蛋白源。

外文摘要：To evaluate the feasibility of cottonseed protein in largemouth bass(Micropterus salmoides)diet,five isonitrogen-isolipid experimental diets(CPC0 as control group,CPC_(3),CPC_(3),CPC_(3),and CPC_(3))were prepared by replacing 30%fish meal with 4 cottonseed proteins of different quality.Micropterus salmoides[average body weight(12.20±0.11)g]were fed in indoor circulating culture system for 8 weeks.The results showed that the nutrient composition,contents of gossypol,raffinose and stysaccharide of the four kinds of cottonseed proteins were different,and CPC_(3)cottonseed protein quality was the best.The final body weight,weight gain rate and specific growth rate of CPC_(3)group were significantly higher than those of the other groups,but there were no significant difference in the routine nutrients and muscle amino acid nutritional composition among all groups.The activities of SOD and GSH-Px and the mRNA expressions of CAT and SOD in liver of CPC_(3)group were the highest,while the content of MDA was the lowest.The relative expressions of anti-inflammatory factors IL-10 and TGF-βwere the highest in CPC_(3)group,while the relative expressions of pro-inflammatory factors IL-1β,IL-8 and TNF-αwere the lowest in CPC_(3)group.In addition,cottonseed protein sources could significantly affect the liver protein metabolism of largemouth bass,and the activities of ALT and AST and the expression levels of P13 K,Akt,mTOR,S6 K1 and 4 E-BP in CPC_(3)group were the highest.Meanwhile,it was found that CPC_(3)group had the highest intestinal T-SOD enzyme activity,the lowest MDA content,and the lowest intestinal permeability indexes(diamine oxidase,D-lactic acid and endotoxin).Cottonseed protein sources also affected the expression of tight junction protein-related genes ZO-1,Claudin-1 and Occludin in the intestine.The results showed that different cottonseed protein quality had different effects on the growth and health of M.salmoides,among which cottonseed protein CPC_(3)showed the best effect and significantly improved the liver and intestinal health of M.salmoides,thereby promoting the growth of M.salmoides.Cottonseed protein CPC_(3)can be used as a good protein source for M.salmoides feed.