文献类型：期刊文献

中文题名：黄芪多糖的糖含量与饮片截面积的关系及对小鼠免疫器官指数的影响

英文题名：Relationship between Sugar Content of Astragalus Polysaccharide and Cross-sectional Area of Decoction Pieces and Its Effect on Immune Organ Index in Mice

作者：彭勤[1];刘晓曦[1]

机构：[1]广东海洋大学滨海农业学院动物医学系,广东湛江524000

年份：2022

卷号：50

期号：11

起止页码：159

中文期刊名：安徽农业科学

外文期刊名：Journal of Anhui Agricultural Sciences

语种：中文

中文关键词：黄芪;多糖;含量;饮片截面积;小鼠;免疫器官指数

外文关键词：Astragalus;Polysaccharide;Content;Cross-sectional area of decoction pieces;Mouse;Immuno-organ index

中文摘要：[目的]以多糖含量来评价黄芪质量,并研究黄芪多糖对小鼠免疫器官指数的影响。[方法]根据黄芪饮片大小编号为1号、2号和3号,先测量其面积,粉碎机粉碎后过20目筛,经过水提醇沉的方法提取多糖,用酶标仪测定黄芪多糖中的糖含量,选用糖含量最高的提取物制作黄芪多糖药液(10 mg/mL);将小鼠随机分成2组,即对照组和黄芪多糖组(0.01 mL/g)给小鼠灌胃7 d,称量小鼠体重,眼球采血,测量其外周血白细胞数及采集其胸腺、脾脏并称重,比较其内脏系数和外周血白细胞数的变化。[结果]1~3号黄芪多糖的提取率分别为5.75%、5.95%和7.76%,糖含量分别为208.75、209.45和212.35 mg/g,黄芪饮片的内圈大小和多糖提取量呈正相关,3号饮片质量最佳;小鼠灌胃黄芪多糖后胸腺、脾脏系数显著增高。[结论]黄芪多糖改善了小鼠的免疫功能。

外文摘要：[Objective]The astragalus quality was evaluated by the polysaccharide content,and the effect of astragalus polysaccharide on the immune organ index of mice was studied.[Method]According to the size of astragalus decoction pieces numbered as No.1,No.2 and No.3,the area of astragalus decoction pieces was measured first.After being crushed by a pulverizer and sieved through 20 meshes,the polysaccharide was extracted by water extraction and alcohol precipitation.The sugar content in astragalus polysaccharide was determined by an enzyme labeling instrument.The extract with the highest sugar content was selected to make astragalus polysaccharide solution(10 mg/mL);The mice were randomly divided into two groups:control group and astragalus polysaccharide group(0.01 mL/g).The mice were gavaged for 7 days.The weight of the mice was weighed,the blood was collected from the eyeball,the number of leukocytes in peripheral blood was measured,and the thymus and spleen were collected and weighed.The changes of visceral coefficient and the number of leukocytes in peripheral blood were compared.[Result]The extraction rates of 1-3 astragalus polysaccharides were 5.75%,5.95%and 7.76%respectively,and the sugar contents were 208.75,209.45 and 212.35 mg/g respectively.There was a positive correlation between the inner ring size of astragalus decoction slices and the extraction amount of polysaccharides,and the quality of No.3 slices was the best.After intragastric administration of astragalus polysaccharides,the coefficients of thymus and spleen increased significantly.[Conclusion]Astragalus polysaccharides improved the immune function of mice.