文献类型：期刊文献

中文题名：不同生长速率高体鰤消化、抗氧化及免疫性能差异

英文题名：Differences in Digestive,Antioxidant,and Immune Performance in Seriola dumerili with Different Growth Rates

作者：李晓净[1];茹笑影[2];黄洋[1,2];邓秋霞[2];陈培培[3];郭玉婷[1];林浩毅[1];朱春华[1,2]

机构：[1]广东海洋大学水产学院/广东省名特优鱼类生殖调控与繁育工程技术研究中心,广东湛江524088;[2]南方海洋科学与工程广东省实验室(湛江),广东湛江524057;[3]中国水产有限公司,北京100160

年份：2026

卷号：46

期号：1

起止页码：56

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：北大核心2023、、北大核心

基金：广东海洋大学自科项目(030301022301);湛江湾实验室人才团队引进项目(ZJW-2023-10);广东省现代化海洋牧场适养品种核心技术攻关项目(2024-MRB-00-001);2024年湛江市科技计划项目(2024R1010);2025年湛江市科技计划项目(2025R02113);广东省“扬帆计划”-2025年现代化海洋牧场产业人才振兴计划子项目(HYJ-YFJH-2025001)。

语种：中文

中文关键词：高体鰤;生长;消化酶;抗氧化酶;非特异性免疫酶;基因表达

外文关键词：Seriola dumerili;growth;digestive enzymes;antioxidant enzymes;non-specific immune enzymes;gene expression

中文摘要：【目的】研究在相同养殖环境下,不同生长速率高体鰤(Seriola dumerili)消化、抗氧化、免疫性能,探究生长速率存在差异的原因。【方法】将相同喂养条件下养殖至1龄的高体鰤分为快生长组和慢生长组,每组随机选择10尾鱼测量其全长、体质量、体高等生长参数;并测定肝脏、胃及肠道中消化酶活性,血清、肝脏中的抗氧化酶和非特异性免疫酶活性,以及肝脏中免疫及抗氧化相关基因的表达水平。【结果】消化酶测定结果显示,快生长组肝脏中胃蛋白酶(PPS)、脂肪酶(LPS)和胰蛋白酶(TPS)活性以及肠道和胃中淀粉酶(AMS)和TPS活性均显著高于慢生长组(P<0.05),而肠道和胃中的PPS活性显著低于慢生长组(P<0.05)。抗氧化酶测定结果显示,快生长组肝脏中的过氧化氢酶(CAT)活性、丙二醛(MDA)含量以及血清中谷胱甘肽过氧化物酶(GPX)、CAT及SOD活性均显著高于慢生长组(P<0.05),而肝脏中超氧化物歧化酶(SOD)活性以及血清中MDA的含量显著低于慢生长组(P<0.05)。非特异性免疫酶活性测定结果显示,快生长组肝脏中谷草转氨酶(AST)和谷丙转氨酶(ALT)活性以及血清中碱性磷酸酶(AKP)和酸性磷酸酶(ACP)活性均显著高于慢生长组(P<0.05),而血清中AST及ALT活性显著低于慢生长组(P<0.05)。qPCR结果显示,快生长组肝脏中抗氧化相关基因gpx1b、cat、sod1、sod3和gss以及免疫相关基因il10和tgfβ2的表达水平均显著上调(P<0.05)。【结论】生长快的高体鰤消化、抗氧化和免疫性能更强,为探讨高体鰤个体间生长速率差异原因提供了理论依据。

外文摘要：【Objective】To investigate the digestive,antioxidant,and immune performance of greater amberjack(Seriola dumerili)with different growth rates under the same aquaculture environment,and explore the reasons behind the differences in growth rates.【Method】The fish that were raised to one year old under identical feeding conditions were categorized into a fast-growth group and a slowgrowth group.Ten fish were randomly selected for each group to measure their total length,body mass,and body height.Additionally,the activities of digestive enzymes in the liver,stomach,and intestine were assessed,along with the activities of antioxidant enzymes and non-specific immune enzymes in serum and liver.Furthermore,the expression of immune-and antioxidant-related genes in the liver were analyzed.【Result】The results of the digestive enzyme assays indicated that the activities of pepsin(PPS),lipase(LPS),and trypsin(TPS)in the liver and of amylase(AMS)and TPS in the intestine and stomach,were significantly elevated compared to those in the fast-growing group(P<0.05).In contrast,PPS activity was significantly lower than that in the slow-growing group(P<0.05).The antioxidant enzyme assay results revealed that the activity of catalase(CAT)and the content of malondialdehyde(MDA)in the liver,as well as the glutathione peroxidase(GPX),CAT,and superoxide dismutase(SOD)in the serum,were significantly higher in the fast-growing group than those in the slow-growing group.Conversely,SOD activity in the liver and MDA content in the serum were significantly lower in the fast-growing group than those in the slow-growing group(P<0.05).Nonspecific enzyme activity assay results showed that aspartate aminotransferase(AST)and alanine aminotransferase(ALT)activities in the liver,as well as alkaline phosphatase(AKP)and acid phosphatase(ACP)activities in the serum,were significantly higher in the fast-growing group than those in the slow-growing group(P<0.05).However,AST and ALT activities in the serum were significantly lower than those in the slow-growing group(P<0.05).The qPCR results indicated that the expression levels of the antioxidant genes gpx1b,cat,sod1,sod3,and gss,as well as the immunerelated genes il10 and tgfβ2,were significantly higher in the fast-growing group(P<0.05).【Conclusion】The fast-growing greater amberjack exhibits enhanced digestive,antioxidant,and immune capabilities,providing a theoretical basis for exploring the reasons behind differences in growth rates among individuals of this species.