文献类型：期刊文献

英文题名：Integrated analyses of characterization and transcriptome reveal the adaptive response mechanism of Bacillus cereus FCHN 7-1 in cadmium adsorption

作者：Chen, Yinyan[1,2];Fang, Zhijia[1,2];Iddrisu, Lukman[2];Ye, Jianzhi[1,2];Pan, Xiaowei[1];Liang, Yaohui[1];Gooneratne, Ravi[3]

机构：[1]Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Coll Food Sci & Technol, Guangdong Prov Engn Technol,Res Ctr Marine Food,Gu, Zhanjiang 524088, Peoples R China;[3]Lincoln Univ, Dept Wine Food & Mol Biosci, Lincoln 7647, Canterbury, New Zealand

年份：2025

卷号：489

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：SCI-EXPANDED(收录号：WOS:001427748100001)、、EI(收录号：20250717875201)、Scopus(收录号：2-s2.0-85217629160)、WOS

基金：This work was supported by the Central Public-interest Scientific Institution Basal Research Fund (NO. 1630122024013) , the National Natural Science Foundation of China (No. 32172215) , and the Natural Science Foundation of Guangdong Province (No. 2024A1515011711) .

语种：英文

外文关键词：Bacillus cereus; Adsorption; Cadmium; Extracellular polymeric substances (EPS); Adaptive response

外文摘要：Cadmium (Cd2+) pollution is a pressing environmental issue that seriously threatens human health. In recent years, microbial extracellular polymeric substances (EPS) have been developed as an eco-friendly and effective solution for heavy metal bioremediation. In this study, Bacillus cereus FCHN 7-1, which was isolated from highly Cd-polluted soils in Hunan Province, China, has strong resistance to Cd2+ and excellent Cd2+ adsorptive capacity. Microscopic analyses showed that B. cereus FCHN 7-1 mainly adsorbed Cd2+ on the surface of EPS via the formation of granule deposits. The Freundlich isotherm was proven to better describe the sorption data with a higher R2 of 0.958, and the pseudo-second-order model fitted the sorption kinetic processes well, with an adsorption capacity of 66.128 mg/g. The variations in zeta potential indicated the occurrence of electrostatic attraction during the Cd2+ adsorption process. XRD, FTIR, 3D-EEM, and XPS analyses revealed that the func- tional groups of the EPS involved in Cd2+ adsorption were mainly C--O, N-H, and COO- groups of proteins in the EPS, which greatly facilitated the adsorption of Cd2+ by forming EPS-metal complexes. Transcriptome sequencing analysis showed that an adaptive response to Cd2+ increased the expression of genes involved in amino acid biosynthesis and metabolism (asnB, alsS, ablA), and ABC transporter pathways (brnQ, abc-f, U2I57_RS23145), which promoted the synthesis of proteins and secretion of abundant EPS. This study pro- vides new insights into the biosorption of Cd2+ for future applications.