文献类型：期刊文献

中文题名：鹅巴氏杆菌病病原分离鉴定与中药的筛选

英文题名：Isolation and identification of pathogen from diseased goose with Pasteurellosisand screening sensitive Chinese herb and their compounds

作者：马驿[1];陈钦旭[1];陈秀眉[1];陈均玉[1];彭金菊[1]

机构：[1]广东海洋大学动物医学系

年份：2011

卷号：47

期号：9

起止页码：49

中文期刊名：中国兽医杂志

外文期刊名：Chinese Journal of Veterinary Medicine

收录：北大核心2008、北大核心

语种：中文

中文关键词：中药;中药复方;巴氏杆菌

外文关键词：Chinese herbs; Chinese medicine formula; Pasteurella multocida

中文摘要：应用常规分离鉴定技术,对湛江市某养鹅场的病鹅进行病原分离与鉴定,通过试管二倍稀释法测定鱼腥草、五倍子、黄芩等30种中药药液对病原菌的最小抑菌浓度(MIC)及最小杀菌浓度(MBC),筛选出抗菌作用较强的中药作为主药,与其他中药组成复方,测定中药复方对病原菌的体外抑制效果。结果表明:分离的病原菌符合巴氏杆菌的生化特性;五倍子(MIC=3.125mg/mL)和诃子(MIC=12.5mg/mL)对巴氏杆菌有较强抑菌活性,选取五倍子、诃子、五味子、乌梅、山茱萸、石榴皮、栀子和虎杖作为主药与其他中药分别组成复方,主药与大多数中药组成的复方抗菌作用增强。

外文摘要：Pathogenic bacteria were isolated from the infected geese which were collected from a goose farm in Zhanjiang by routine bacterial isolation and identification technology.Moreover,the minimal inhibitory concentration（MIC） and minimum bactericidal concentration（MBC） of 30 kinds of Chinese herbs affected on pathogenic bacteria were measured,such as Herba Houttuyniae,Galla Chinensis,Radices Scutellaria,etc,and the antibacterial effects of other Chinese herbs combined with the selected Chinese herbs which had the significant inhibitory effects on pathogenic bacteria were measured.The results show that the biochemical character of the isolated pathogenic bacteria were same as those of Pasteurella multocida,and the Chinese herbs such as Galla Chinensis（MIC=3.125 mg/mL） and Fructus Chebulae（MIC=12.5 mg/mL） had significant inhibitory effects on Pasteurella multocida,the inhibitory effects were improved when Galla Chinensis,Fructus Chebulae,Ffructus Schizandrae,Fructus Mume,Fructus Corni,Pericarpium Granati,Fructus Gardeniae and Rhizoma Polygoni Cuspidati were combined with many other Chinese herbs.