文献类型：期刊文献

英文题名：Effects of dietary probiotic supplementation on the growth, gut health and disease resistance of juvenile Nile tilapia (Oreochromis niloticus)

作者：Xia, Yun[1];Wang, Miao[1];Gao, Fengying[1];Lu, Maixin[1];Chen, Gang[2]

机构：[1]Chinese Acad Fishery Sci, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr, Pearl River Fisheries Res Inst, Guangzhou 510380, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524025, Peoples R China

年份：2020

卷号：6

期号：1

起止页码：69

外文期刊名：ANIMAL NUTRITION

收录：SCI-EXPANDED(收录号：WOS:000520511900010)、、WOS

基金：This work was supported by the China Agricultural Research System (CARS-46) and Science and Technology Program of Guangzhou, China (grant No.: 201707010312). We would like to thank Professor Xiaoying Hu at the South China Botanical Garden, Chinese Academy of Sciences, for the intestinal electron microscopy work in this study.

语种：英文

外文关键词：Tilapia; Bacillus subtilis; Bacillus cereus; Intestinal microbiota; Probiotic

外文摘要：This study investigated the effects of the Streptococcus agalactiae antagonizing probiotics Bacillus cereus NY5 and Bacillus subtilis as feed additives for Nile tilapia in terms of growth performance, intestinal health and resistance to S. agalactiae. A total of 720 apparently healthy juvenile Nile tilapia (0.20 +/- 0.05 g) were randomly divided into 4 equal groups with 3 replicates for each group. Fish were fed a basal diet (control check group, CK group) supplemented with B. subtilis (1 x 10(8) CFU/g feed, BS group), B. cereus NY5 (1 x 10(8) CFU/g feed, BC group), and B. subtilis thorn B. cereus NY5 (0.5 x 10(8) CFU/g feed of each probiotic, BS thorn BC group) for 6 wk, and the probiotic supplementation groups were then fed the basal diet for 1 wk to investigate the gut microbial community. The results of this study showed that BS thorn BC and BC treatments significantly increased weight gain (WG), feed conversion ratio (FCR) and S. agalactiae resistance in Nile tilapia (P < 0.05). Gut microvilli length and density and c-type lysozyme (lyzc) gene expression were significantly increased by probiotic supplementation (P < 0.05). The results of high-throughput sequencing showed that the B. cereus NY5 and B. subtilis thorn B. cereus NY5-supplemented feed resulted in a significant improvement in tilapia autochthonous gut bacterial communities and had a stimulation effect on a variety of potential probiotics after 6 wk of feeding. After cessation of probiotic administration for 1 wk, the gut bacteria of the fish in the BS thorn BC and BC groups had minor changes and maintained a stable state. Consequently, it was inferred that, as a feed supplement, B. cereus NY5 and the mixture of B. subtilis and B. cereus NY5 at 1 x 10(8) CFU/g feed were able to promote growth and disease resistance, which may be associated with the supplement's effects on gut immune status, intestinal morphology, and intestinal microbial community composition. (c) 2019, Chinese Association of Animal Science and Veterinary Medicine. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.