文献类型：期刊文献

中文题名：牡蛎酶解产物改善睡眠作用效果研究

英文题名：Effects of enzymatic hydrolysis products of oyster on sleeping improving

作者：张婷[1];秦小明[1,2];章超桦[1,2];曹文红[1,2];郑惠娜[1,2];高加龙[1,2];林海生[1,2]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]广东省水产品加工与安全重点实验室,广东普通高等学校水产品深加工重点实验室,国家贝类加工技术研发分中心(湛江),南海生物资源开发与利用协同创新中心,广东湛江524088

年份：2021

卷号：36

期号：3

起止页码：430

中文期刊名：大连海洋大学学报

外文期刊名：Journal of Dalian Ocean University

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：财政部和农业农村部:国家现代农业产业技术体系资助(CARS-49)。

语种：中文

中文关键词：牡蛎;酶解产物;改善睡眠;神经递质

外文关键词：Crassostrea hongkongensis;enzymatic hydrolysis product;improve sleep;neurotransmitter

中文摘要：为探究香港牡蛎Crassostrea hongkongensis酶解产物(enzymatic products of oysters,EPO)改善睡眠的作用效果,将试验小鼠分为阴性对照组(灌胃等体积蒸馏水)、阳性对照组(灌胃地西泮2 mg/kg体质量)及EPO低、中、高剂量组(分别灌胃250、500、1000 mg/kg体质量,分别记为EPO-L、EPO-M、EPO-H),每天灌胃一次,连续灌胃30 d后进行直接睡眠试验、延长戊巴比妥钠睡眠时间试验、戊巴比妥钠阈下剂量催眠试验和戊巴比妥钠潜伏期试验,并采用ELISA试剂盒检测小鼠下丘脑和大脑皮层中5-羟色胺(serotonin,5-HT)、γ-氨基丁酸(gamma aminobutyric acid,GABA)、褪黑素(melatonin,MT)、去甲肾上腺素(norepinephrine,NE)和多巴胺(dopamine,DA)的含量。结果表明:牡蛎酶解产物3个剂量组均无直接睡眠作用,但与阴性对照组相比,均能显著延长小鼠睡眠时间(P<0.05),缩短睡眠潜伏期(P<0.05),且EPO中剂量组能显著提高小鼠入睡率(P<0.05);与阴性对照组相比,EPO低、中剂量组均能显著提高下丘脑5-HT、GABA含量(P<0.05),且显著降低下丘脑DA、NE含量(P<0.05),高剂量组能显著降低下丘脑DA含量(P<0.05);EPO低剂量组能显著提高大脑皮层GABA含量(P<0.05),中剂量组能显著提高大脑皮层GABA、MT含量(P<0.05),且显著降低大脑皮层DA含量(P<0.05),高剂量组能显著提高大脑皮层MT含量(P<0.05),且显著降低大脑皮层DA、NE含量(P<0.05)。研究表明,牡蛎酶解产物通过提高脑内抑制性神经递质5-HT、GABA、MT含量,降低兴奋性神经递质DA、NE含量,缩短睡眠潜伏期,提高睡眠发生率,延长睡眠时间,起到改善睡眠的作用,且灌胃EPO中剂量500 mg/kg体质量时改善睡眠效果最佳。

外文摘要：In order to explore the effect of enzymatic products of oysters(EPO)on sleep improving,the experiment mice were divided into negative control group(equal volume of distilled water by gavage),positive control group(diazepam 2 mg/kg body mass by gavage)and low,medium and high dose EPO groups(gavage EPO 250,500 and 1000 mg/kg body mass,respectively,denoted as EPO-L,EPO-M and EPO-H).After gavage once a day for 30 days,direct sleep experiment,prolonged pentobarbital sodium sleep time experiment,pentobarbital sodium subthreshold dose hypnosis experiment and incubation period experiment of barbital sodium were performed,the ELISA kit were to detect the contents of serotonin(5-HT),gamma-aminobutyric acid(GABA),melatonin(MT),norepinephrine(NE)and dopamine(DA)in mice hypothalamus and cerebral cortex.The results showed that significant prolongation of the sleep time(P<0.05),and shortening the sleep latency(P<0.05)were observed in the mice in the three doses of EPO groups compared with that in the negative control group,without direct sleep effect.There was significant increase in the sleeping rate of the mice in the medium dose of EPO group compared with that in the negative control group(P<0.05).The mice in the low and medium dose of EPO groups had significant increase in the contents of hypothalamic 5-HT and GABA(P<0.05),and significant decrease in the contents of hypothalamic DA and NE(P<0.05)compared with the mice in the negative control group did,with significant decrease in the hypothalamus DA content in the high dose group(P<0.05).The contents of GABA in cerebral cortex were significantly increased in low dose EPO group(P<0.05),the contents of GABA and MT in cerebral cortex were significantly increased,and the content of DA in cerebral cortex was significantly decreased in medium dose EPO group(P<0.05).The significant increase in MT content of cerebral cortex,and significant decrease in DA and NE contents of cerebral cortex(P<0.05)were found in the high dose group.The findings indicated that EPO led to improve sleep by increasing the contents of inhibitory neurotransmitters 5-HT,GABA and MT in the brain,by reducing the contents of excitatory neurotransmitters DA and NE,by shortening the sleep latency period,and by increasing the incidence of sleep and prolonging sleep time.