文献类型：期刊文献

英文题名：Integrative Transcriptomic and Small RNA Sequencing Reveals Immune-Related miRNA-mRNA Regulation Network for Soybean Meal-Induced Enteritis in Hybrid Grouper,Epinephelus fuscoguttatus female xEpinephelus lanceolatus male

作者：He, Yuanfa[1,2,3];Ye, Guanlin[1,2,3];Chi, Shuyan[1,2,3,4];Tan, Beiping[1,2,3];Dong, Xiaohui[1,2,3];Yang, Qihui[1,2,3];Liu, Hongyu[1,2,3];Zhang, Shuang[1,2,3]

机构：[1]Guangdong Ocean Univ, Lab Aquat Anim Nutr & Feed, Fisheries Coll, Zhanjiang, Peoples R China;[2]Aquat Anim Precis Nutr & High Efficiency Feed Eng, Zhanjiang, Peoples R China;[3]Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Tech, Zhanjiang, Peoples R China;[4]Guangdong Ocean Univ, Southern Marine Sci & Engn Guangdong Lab, Zhanjiang, Peoples R China

年份：2020

卷号：11

外文期刊名：FRONTIERS IN IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000563324000001)、、WOS

基金：This study was financially supported by the National Key R&D Program of China (2019YFD0900200), the China Agriculture Research System (CARS-47), the National Natural Science Foundation of China (NSFC 31772864), and the Fund of Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) (ZJW-2019-06).

语种：英文

外文关键词：hybrid grouper; inflammation; RNA-seq; intestinal health; immune response

外文摘要：A 10-week feeding experiment was conducted to reveal the immune mechanism for soybean meal-induced enteritis (SBMIE) in hybrid grouper, Epinephelus fuscoguttatus female x Epinephelus lanceolatus male. Four isonitrogenous and isolipidic diets were formulated by replacing 0, 10, 30, and 50% fish meal protein with soybean meal (namely FM, SBM10, SBM30, and SBM50, respectively). The weight gain rate of the SBM50 group was significantly lower than those of the other groups. Plica height, muscular layer thickness, and goblet cells of the distal intestine in the SBM50 group were much lower than those in the FM group. The intestinal transcriptomic data, including the transcriptome and miRNAome, showed that a total of 6,390 differentially expressed genes (DEGs) and 92 DEmiRNAs were identified in the SBM50 and FM groups. DEmiRNAs (10 known and 1 novel miRNAs) and their DE target genes were involved in immune-related phagosome, natural killer cell-mediated cytotoxicity, Fc gamma R-mediated phagocytosis, and the intestinal immune network for IgA production pathways. Our study is the first to offer transcriptomic and small RNA profiling for SBMIE in hybrid grouper. Our findings offer important insights for the understanding of the RNA profile and further elucidation of the underlying molecular immune mechanism for SBMIE in carnivorous fish.