文献类型：期刊文献

英文题名：Multi-marker eDNA metabarcoding reveals more abundant coral biodiversity in a subtropical coastal reef ecosystem

作者：Hong, Miao[1,2];Duan, Xiaowei[3];Chen, Yimeng[1,2];Wang, Jianjia[2,5];Zhou, Xijie[2];Wang, Guoxian[2];Zhang, Jingjing[2];Liu, Senkai[2];Shi, Wenjie[2];Luo, Zhaohe[2,4];Zheng, Xinqing[2,5,6]

机构：[1]Nanjing Univ Informat Sci & Technol, Sch Marine Sci, Nanjing 210044, Peoples R China;[2]Minist Nat Resources, Inst Oceanog 3, Key Lab Marine Ecol Conservat & Restorat, Xiamen 351005, Peoples R China;[3]Tsinghua Shenzhen Int Grad Sch, Shenzhen 518071, Peoples R China;[4]Xiamen Univ Technol, Sch Environm Sci & Engn, Xiamen 361024, Peoples R China;[5]Minist Nat Resources, Observat & Res Stn Isl & Coastal Ecosyst Western T, Xiamen 361005, Peoples R China;[6]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518116, Peoples R China

年份：2026

卷号：213

外文期刊名：MARINE ENVIRONMENTAL RESEARCH

收录：SCI-EXPANDED(收录号：WOS:001615586300004)、、WOS

基金：This work was financially supported by the National Key Research and Development Program of China (2022-20) , the Scientific Research Foundation of the Third Institute of Oceanography, Ministry of Natural Resources of China (2025044, 2019017) , the Sustainable Development Project of Shenzhen (KCXFZ20211020165547011) , the National Natu-ral Science Foundation of China (42376110) , and the Fujian Provincial Natural Science Funds for Distinguished Young Scholar (2023J06043) .

语种：英文

外文关键词：Environmental DNA (eDNA); Multi-marker metabarcoding; Scleractinian corals; Coral reef biodiversity; ITS2 and 12S; Biodiversity monitoring

外文摘要：Environmental DNA (eDNA) metabarcoding has emerged as a powerful tool for monitoring biodiversity, particularly for taxa that are difficult to survey visually. Our study applied a multi-marker environmental DNA (eDNA) metabarcoding approach targeting the nuclear (ITS2) and mitochondrial (12S) genes to assess scleractinian coral diversity in the coastal waters of eastern Shenzhen, China. Compared to traditional diver-based surveys, the eDNA method detected more genera (42 versus 23) and species (77 versus 63), with 55 % of the genera detected by eDNA overlapping with those recorded during visual surveys, thus demonstrating the reliability and advantages of the eDNA approach. Spatial analyses identified offshore biodiversity as "hot-spots" with higher coral diversity and minimal anthropogenic disturbance, whereas urbanized nearshore areas formed "cold spots". Seasonal sampling revealed the highest detection rates in autumn, likely reflecting environmental conditions that enhance eDNA persistence. Water temperature, salinity, and nutrient concentrations strongly influence coral eDNA abundance and shape detection patterns. Although methodological challenges such as incomplete reference databases and primer biases remain, our findings demonstrate that multi-marker eDNA metabarcoding provides a sensitive, scalable, and complementary tool for coral biodiversity monitoring. Expanding reference databases and refining assay methods will enhance the accuracy and conservation relevance of eDNA-based assessments, particularly in managing coral ecosystems in rapidly urbanizing coastal regions.