文献类型：期刊文献

英文题名：A Chromosome-Level Genome Assembly of the Spotted Scat (Scatophagus argus)

作者：Huang, Yuanqing[1];Mustapha, Umar Farouk[1];Huang, Yang[1];Tian, Changxu[1];Yang, Wei[1,2];Chen, Huapu[1];Deng, Siping[1];Zhu, Chunhua[1,3];Jiang, Dongneng[1];Li, Guangli[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Guangdong Res Ctr Reprod Control & Breeding Techn, Zhanjiang, Peoples R China;[2]Shantou Univ, Guangdong Prov Key Lab Marine Biotechnol, Shantou, Guangdong, Peoples R China;[3]Southern Marine Sci & Engn Guangdong Lab Zhanjian, Zhanjiang, Guangdong, Peoples R China

年份：2021

卷号：13

期号：6

外文期刊名：GENOME BIOLOGY AND EVOLUTION

收录：SCI-EXPANDED(收录号：WOS:000670919400012)、、WOS

基金：This study was supported by grants from the Key Project of "Blue Granary Science and Technology Innovation" of the Ministry of Science and Technology (2018YFD0901203); Natural Science Foundation of Guangdong Province (2018B030311050); Guangdong Basic and Applied Basic Research Foundation (2019A1515012042 and 2021A1515010430); Independent Project of Guangdong Province Laboratory (ZJW-2019-06); grant from the Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams (2019KJ149); Department of Education of Guangdong Province (2018KTSCX090); Program for Scientific Research Start-Up Funds of Guangdong Ocean University.

语种：英文

外文关键词：spotted scat; genomics; PacBio sequencing; Hi-C proximity mapping; chromosomal assembly

外文摘要：The spotted scat, Scatophagus argus is a member of the family Scatophagidae found in Indo-Pacific coastal waters. It is an emerging commercial aquaculture species, particularly in East and Southeast Asia. In this study, the first chromosome-level genome of S. argus was constructed using PacBio and Hi-C sequencing technologies. The genome is 572.42 Mb, with a scaffold N50 of 24.67 Mb. Using Hi-C data, 563.28 Mb (98.67% of the genome) sequences were anchored and oriented in 24 chromosomes, ranging from 12.57 Mb to 30.38 Mb. The assembly is of high integrity, containing 94.26% conserved single-copy orthologues, based on BUSCO analysis. A total of 24,256 protein-coding genes were predicted in the genome, and 96.30% of the predicted genes were functionally annotated. Evolutionary analysis showed that S. argus diverged from the common ancestor of Japanese puffer (Takifugu rubripes) approximately 114.8 Ma. The chromosomes of S. argus showed significant correlation to T. rubripes chromosomes. A comparative genomic analysis identified 49 unique and 90 expanded gene families. These genomic resources provide a solid foundation for functional genomics studies to decipher the economic traits of this species.