文献类型：期刊文献

英文题名：A novel deletion in KRT75L4 mediates the frizzle trait in a Chinese indigenous chicken

作者：Dong, Jing[1];He, Chuan[2];Wang, Zhibing[1];Li, Yanqing[1];Li, Shanshan[1];Tao, Lin[1];Chen, Jiebo[1];Li, Donghua[1];Yang, Fenxia[1];Li, Naibin[1];Zhang, Quan[1];Zhang, Li[1];Wang, Guangqin[3];Akinyemi, Fisayo[2];Meng, He[2];Du, Bingwang[1]

机构：[1]Guangdong Ocean Univ, Coll Agr, Dept Anim Sci, Zhanjiang 524088, Guangdong, Peoples R China;[2]Shanghai Jiao Tong Univ, Shanghai Key Lab Vet Biotechnol, Sch Agr & Biol, Dept Anim Sci, Shanghai 200240, Peoples R China;[3]Zhanjiang Jinsheng Anim Husb Sci & Technol Ltd, Zhanjiang 524025, Guangdong, Peoples R China

年份：2018

卷号：50

外文期刊名：GENETICS SELECTION EVOLUTION

收录：SCI-EXPANDED(收录号：WOS:000454127400001)、、WOS

基金：This study was supported by program grant funding from a key project of Zhanjiang modern agriculture (2016A03010): "Strain breeding and molecular mechanism research on resistance to high temperature characteristics of Kirin chicken (frizzle fowl)". The funding bodies had no role neither in the design of the study, nor in the collection, analysis, interpretation of data, and writing the manuscript.

语种：英文

外文摘要：BackgroundHighly diversified in morphology and structure, feathers have evolved into various forms. Frizzle feathers, which result from a developmental defect of the feather, are observed in several domestic chicken breeds. The frizzle phenotype is consistent with incomplete dominance of a major gene, but the molecular mechanisms that underlie this phenotype remain obscure. Kirin, a Chinese indigenous chicken breed that originated in the Guangdong province, is famous for its frizzle feathers. The KRT75 gene is considered as the dominant gene responsible for the frizzle trait in several chicken breeds, but this is not the case in the Kirin breed. Thus, the objective of our study was to investigate the genomic region and mutation responsible for this phenotype in this particular breed.ResultsA resource population was produced by crossing Kirin and Huaixiang chickens to produce F-1 and F-2 generations. DNA samples from 75 frizzle feather and normal feather individuals were sequenced with double-digest genotyping by sequencing (dd-GBS). After the detection of 525,561 high-quality variants, a genome-wide association analysis was carried out and the gene responsible for the frizzle phenotype was localized within the type II -keratin cluster on chromosome 33. Sanger sequencing was used to screen for mutations in the exons of five genes of this type II -keratin cluster. A 15-bp deletion in exon 3 of KRT75L4 that showed complete segregation with the frizzle phenotype was detected within the F-2 population. Transcriptome sequencing demonstrated that KRT75L4 was expressed but that the transcript was shorter in Kirin than in Huaixiang chickens. In addition, by using Sanger sequencing, we were able to confirm that the deletion was in complete linkage with frizzle feathers.ConclusionsA deletion in the KRT75L4 gene is responsible for the frizzle feather phenotype in the Kirin chicken. The identification of this mutation, which causes a developmental defect of avian integument appendages, will improve our understanding of the mechanisms that are involved in feather formation.