文献类型：期刊文献

中文题名：Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach

英文题名：Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach

作者：王辉[1];牛景彦[2];刘佳慧[3];杨洪帅[3];刘志刚[3]

机构：[1]Life Sciences College, Huaiyin Normal University;[2]Polytechnic Department, Jiaozuo Teachers College;[3]Fisheries College, Guangdong Ocean University

年份：2017

卷号：35

期号：3

起止页码：537

中文期刊名：Chinese Journal of Oceanology and Limnology

外文期刊名：中国海洋湖沼学报（英文版）

收录：SCI-EXPANDED(收录号：WOS:000399168400007)、CSTPCD、、Scopus、WOS、CSCD2017_2018、CSCD

基金：Supported by the Guangdong Province Science and Technology Project (No. 2010B020201014), the Guangdong Province South China Sea Invertebrates Healthy Culture Engineering Technology Research Center (No. GCZX-A0909), the Guangdong Province Ocean and Fisheries Science and Technology Extension Project (No. 20120980), the Guangdong Province Industry-University-Science Partnership Project (No. 20110908), and the Sci & Tech Plan of Huaiyin Normal University (No. WH0031)

语种：中文

中文关键词：Tetraselmis subcordiformis; specific growth; temperature; salinity; pH; growth model

外文关键词：模型建设;响应面法;绿藻;比生长速率;实验室条件;中心组合设计;线性效应;交互作用

中文摘要：The green alga Tetraselmis subcordiformis has been widely used as a quality live food for aquaculture species, and also has been studied as a model organism for the photo-biological production of hydrogen. We attempted to quantify the relationship between T. subcordiformis specific growth rate （SGR） and three important environmental factors （temperature, salinity, and pH） using the central composite design and response surface method under laboratory conditions. The results showed that the linear effects of temperature and salinity were significant （P〈0.05）, and they were equally important in impacting T. subcordiformis specific growth; the linear effect of pH was not significant （P〉0.05）; the interactive effect of temperature and pH was significant （P〈0.05）, whereas the temperature ~ salinity and salinity x pH interactions were not significant （P〉0.05）; all of the quadratic effects of the three factors were significant （P〈0.05）. A model equation for specific growth rate with the three factors was established, with the unadjusted and predictive R2 as high as 0.990 and 0.921, respectively, suggesting that the model was a very good fit and that it could be used to predict SGR. Through optimizing the reliable model, an optimal 3-factor combination of 25~C/35 of salinity/pH 7.9 was obtained, at which the maximum specific growth rate （0.65） was recorded, with a desirability value of 93.8%. These experimental results could serve as guidelines for increasing T. subcordiformis production efficiency.

外文摘要：The green alga Tetraselmis subcordiformis has been widely used as a quality live food for aquaculture species, and also has been studied as a model organism for the photo-biological production of hydrogen. We attempted to quantify the relationship between T. subcordiformis specific growth rate(SGR) and three important environmental factors(temperature, salinity, and p H) using the central composite design and response surface method under laboratory conditions. The results showed that the linear effects of temperature and salinity were significant( P< 0.05), and they were equally important in impacting T. subcordiformis specific growth; the linear effect of p H was not significant( P >0.05); the interactive effect of temperature and p H was significant( P <0.05), whereas the temperature ′ salinity and salinity ′ p H interactions were not significant( P >0.05); all of the quadratic effects of the three factors were significant( P< 0.05). A model equation for specific growth rate with the three factors was established, with the unadjusted and predictive R^2 as high as 0.990 and 0.921, respectively, suggesting that the model was a very good fit and that it could be used to predict SGR. Through optimizing the reliable model, an optimal 3-factor combination of 25 ° C/35 of salinity/p H 7.9 was obtained, at which the maximum specific growth rate(0.65) was recorded, with a desirability value of 93.8%. These experimental results could serve as guidelines for increasing T. subcordiformis production efficiency.