文献类型：期刊文献

中文题名：螯合硒对牡蛎肽免疫活性的影响

英文题名：Chelation of Oyster Peptides with Selenium and Its Impact on Their Immune Activity

作者：杨君扬[1];张烘煜[1];陈忠琴[1];谭明堂[1];林海生[1];高加龙[1];郑惠娜[1];曹文红[1];杨维[1,2];陈忆宾[2]

机构：[1]广东海洋大学食品科技学院,国家贝类加工技术研发分中心(湛江),广东湛江524088;[2]海南翔泰渔业股份有限公司,海南澄迈571900

年份：2025

卷号：45

期号：1

起止页码：106

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：北大核心2023、、北大核心

基金：国家贝类产业技术体系(CARS-49);国家重点研发计划课题(2018YFD0901105)。

语种：中文

中文关键词：牡蛎肽;硒螯合物;特征肽;RAW264.7细胞;免疫活性

外文关键词：oyster peptide;selenium chelate;characterized peptide;RAW264.7 cells;immunore activity

中文摘要：【目的】通过螯合结构优化实现牡蛎肽硒复合物免疫活性功能的提升。【方法】借助Capto Q离子交换层析对牡蛎肽及其硒螯合物进行分离纯化,筛选出易与硒结合的肽;通过红外和圆二色光谱对比螯合前后官能团的变化确定硒的结合位点;运用液相色谱-串联质谱联用(LC-MS/MS)和分子对接对硒螯合肽结构特征及结合位点进行分析;以RAW264.7巨噬细胞为实验对象,对比牡蛎肽及其硒螯合物洗脱组分(P2和F2)的细胞免疫应答变化。【结果】波谱和分子对接结果均表明,肽链上的氨基、羧基、羰基等均参与硒离子螯合过程的共价反应,且螯合反应使肽链的二级结构从无规则趋向规则的折叠、转角结构;LC-MS/MS结果表明,在所检测出的螯合物中,相对分子质量越大,碱性氨基酸占比和等电点(PI)值越高的肽链具有更强的硒螯合能力。体外细胞实验表明,F2能显著提高RAW264.7细胞一氧化氮(NO)、活性氧(ROS)、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL-6)的分泌;增加巨噬细胞的吞噬活性;促进免疫相关蛋白COX-2和iNOS的表达,且效果显著优于P2。【结论】牡蛎肽在与硒螯合后结构发生变化,PI值越大的肽更易于与硒发生螯合,螯合后免疫活性得到显著增强。

外文摘要：【Objective】To improve the immune activity function of oyster peptide selenium complex by optimizing chelation structure.【Methods】Oyster peptides and their selenium chelates were separated and purified with the aid of Capto Q ion exchange chromatography to screen for peptides that readily bind selenium.Selenium binding sites were determined by infrared and circular dichroism spectroscopy comparing changes in functional groups before and after chelation.Liquid chromatography-tandem mass spectrometry(LC-MS/MS)and molecular docking were used to analyze the structural features and binding sites of selenium chelating peptides.Finally,RAW264.7 macrophages were used to compare the changes in cellular immune responses of oyster peptides and their selenium chelate-eluting fractions(P2 and F2).【Results】Both spectral and molecular docking results indicated that the amino group,carboxyl group and carbonyl group on the peptide chain were all involved in the covalent reaction of the selenium ion chelation process,and the chelation reaction made the secondary structure of the peptide chain converge from an irregular to a regular folding and corner structure.The results of LC-MS/MS indicated that among the chelates detected,peptide chains with higher relative molecular mass and higher percentages of basic amino acids and PI values had stronger selenium chelating ability.In vitro cellular experiments showed that F2 significantly increased the secretion of nitric oxide(NO),reactive oxygen species(ROS),tumor necrosis factorα(TNFα),and interleukin(IL-6)in RAW264.7 cells;increased phagocytosis activity of macrophages;and promoted the expression of immune-related proteins,COX-2 and iNOS,and the effect was significantly better than that of P2.【Conclusion】The oyster peptides changed structure after being chelated with selenium,and peptides with larger PI values were easier to chelate with selenium,and the immunoreactivity was significantly enhanced.