文献类型：期刊文献

英文题名：Preparation and Characterization of Grouper Bone Peptides-Calcium Complex by Lactic Acid Bacteria Fermentation

作者：Liu, Shouchun[1,2]; Liu, Meijiao[1]; Li, Zhuyi[1]; Chen, Qiuhan[1]; Yang, Xuebo[1]; Chen, Jing[1]; Zhang, Luyao[2]; Zhong, Saiyi[1]; Hong, Pengzhi[1,2]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang, 524088, China; [2] Southern Marine Science and Engineering Guangdong Laboratory[Zhanjiang], Zhanjiang, 524004, China

年份：2024

外文期刊名：SSRN

收录：EI(收录号：20240109860)

语种：英文

外文关键词：Absorption spectroscopy - Bacteria - Calcium compounds - Enzymatic hydrolysis - Fermentation - Fluorescence spectroscopy - Fourier transform infrared spectroscopy - Lactic acid

外文摘要：In this study, a grouper bone peptides-calcium complex (GPs-Ca) was obtained from grouper bone through enzymatic hydrolysis by lactic acid bacteria fermentation. Response surface methodology (RSM) optimization was used to determine the optimal fermentation conditions (fermentation for 75h at 37°C with 4% lactic acid bacteria and 4.5% glucose), the soluble calcium content was 5953.61 mg/L, and the polypeptides content was 21.75 mg/mL. The results of ultraviolet–visible absorption spectroscopy (UV) and fluorescence spectroscopy (Fs) showed a significant spectral shift for GPs-Ca relative to grouper bone peptides (GPs), indicating that the polypeptide and calcium produced new substances after fermentation. FTIR results showed that carboxyl groups participated in the formation of peptides-calcium complex. While, SEM results revealed that calcium ions were embedded or adsorbed on the polypeptide surface. According to the stability analysis, CPs-Ca was stable during heating, alkaline environment and intestinal digestion, but easily dissociated in acidic environment. The above study on the preparation of GPs-Ca by lactobacillus fermentation has the potential to be used as a new type of calcium supplement and provides a scientific basis for the high-value utilization of grouper bone. ? 2024, The Authors. All rights reserved.