文献类型：期刊文献

英文题名：Protection against Vibrio alginolyticus in crimson snapper Lutjanus erythropterus immunized with a DNA vaccine containing the ompW gene

作者：Cai, Shuang-Hu[1,2,3];Lu, Yi-Shan[1,2,3];Jian, Ji-Chang[1,2,3];Wang, Bei[1,2,3];Huang, Yu-Cong[1,2,3];Tang, Ju-Fen[1,2,3];Ding, Yu[1,2,3];Wu, Zao-He[2,3,4]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China;[3]Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524088, Peoples R China;[4]Zhongkai Univ Agr & Engn, Guangzhou 510225, Guangdong, Peoples R China

年份：2013

卷号：106

期号：1

起止页码：39

外文期刊名：DISEASES OF AQUATIC ORGANISMS

收录：SCI-EXPANDED(收录号：WOS:000324887300005)、、WOS

基金：This work was supported by the National Program on Key Basic Research Project (No. 2009CB118704), the 973 Program Earlier Research Project (No. 2011CB111601), the National Science Foundation of Guangdong Province (No. S2011010000257), and the Science and Technology Planning Project of Guangdong Province (No. 2009B050700040) in the People's Republic of China. We thank all the people who have dedicated time to the experiments.

语种：英文

外文关键词：Vibriosis; Vaccination; Recombinant plasmid; Outer membrane protein; pcDNA; Aquaculture

外文摘要：The outer membrane proteins of Vibrio alginolyticus play an important role in the virulence of the bacterium and are potential candidates for vaccine development. In the present study, the ompW gene was cloned, expressed and purified. A DNA vaccine was constructed by inserting the ompW gene into a pcDNA plasmid. Crimson snapper Lutjanus erythropterus (Bloch) were injected intramuscularly with the recombinant plasmid pcDNA-ompW. The expression of the DNA vaccine was detected in gill, head kidney, heart, liver, spleen and injection site muscle of crimson snapper by RT-PCR 7 and 28 d post-vaccination. The ELISA results demonstrated that the DNA vaccine produced an observable antibody response in all sera of the vaccinated fish. In addition, crimson snapper immunized with the DNA vaccine showed a relative percentage survival (RPS) of 92.53%, indicating effective protection against V. alginolyticus infection.