文献类型：期刊文献

英文题名：The influence of PIK3R1 and its phosphorylation on the transplantation immunity of Pinctada fucata martensii

作者：Yin, Guiling[1];Chen, Jie[1];Liang, Haiying[1,2];Ma, Lining[1];Lei, Runlin[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Guangdong, Peoples R China

年份：2026

卷号：613

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:001621413800001)、、WOS

基金：Funding This work was supported by grants from the National Natural Science Foundation of China (No. 31472306) , Guangdong Basic and Applied Basic Research Foundation (No. 2023A1515012924 and No. 2021A1515010962) .

语种：英文

外文关键词：Pinctada fucata martensii; Phosphorylation modification; PI3K/AKT signaling pathway

外文摘要：Pinctada fucata martensii is the main shellfish in marine pearl culture in China. Immune rejection and high mortality after transplantation are the key problems restricting the development of the pearl industry. Previous phosphoproteomics data have shown that nuclear implantation significantly up-regulates PIK3R1 phosphorylation in the PI3K/AKT signaling pathway, suggesting that it may be involved in immune regulation. In this study, the cloned PIK3R1 gene of P. f. martensii (PmPIK3R1) was 2671 bp long, encoding 738 amino acids. The gene contained SH2, RhoGAP, and C1 domains and was highly expressed in the gill and hepatopancreas. The simulated phosphorylation mutant, rPmPIK3R1, was constructed using site-directed mutagenesis, and the protein was successfully purified through a prokaryotic expression system. The results demonstrated that injecting rPmPIK3R1 significantly up-regulated the expression of key genes in the PI3K/AKT signaling pathway (PmPI3K and PmAKT). Additionally, rPmPIK3R1 injection enhanced acid phosphatase activity and significantly reduced the level of apoptosis in gill tissue cells. Furthermore, the injection significantly increased the survival rates at 45 and 60 days compared to the PBS control group. Phosphorylation modification of PmPIK3R1 can inhibit apoptosis by activating the PI3K/AKT pathway, thereby enhancing the immune activity of P. f. martensii after nuclear implantation. This result offers a new perspective on analyzing the immune mechanism of P. f. martensii following nuclear implantation and provides a theoretical basis for enhancing the survival rate of pearl culture.