文献类型：期刊文献

英文题名：Wnt4 in protogynous hermaphroditic orange-spotted grouper (Epinephelus coioides): Identification and expression

作者：Chen, Huapu[1,2,3];Li, Shuisheng[1,2];Xiao, Ling[1,2];Zhang, Yong[1,2];Li, Guangli[3];Liu, Xiaochun[1,2];Lin, Haoran[1,2,4]

机构：[1]Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Inst Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China;[2]Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China;[3]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[4]Hainan Univ, Coll Ocean, Haikou 570228, Hainan, Peoples R China

年份：2015

卷号：183

起止页码：67

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000353087300008)、、WOS

基金：This project was supported by the National 863 Program of China (Nos. 2012AA092201, 2012AA10A407 and 2012AA10A414), Specialized Research Fund for the Doctoral Program of Higher Education (20120171110013), Special Fund for Agro-scientific Research in the Public Interest (201403011), National Natural Science Foundation of China (U1401213), and the Hainan Provincial Science and Technology Program (No. XH201301)).

语种：英文

外文关键词：Wnt4; Embryonic development; Ovarian development; Orange-spotted grouper

外文摘要：Wnt4 (Wingless-type MMTV integration site family member 4) has been demonstrated to play critical roles in ovarian development in mammals, but its function in fish reproduction is still unclear. In the present study, two full-length wnt4 cDNA sequences (named wnt4a and wnt4b) were cloned from the orange-spotted grouper (Epinephelus coioides). Amino add alignment analysis showed that both orange-spotted grouper Wnt4s proteins had the typical characteristics of the Wnt family. RT-PCR revealed that both wnt4a and wnt4b were highly expressed in the ovaries of the orange-spotted grouper. Temporal expression profiles of both wnt4 genes during embryonic and ovarian development were examined. The expressions of wnt4a and wnt4b genes were first detected at the embryonic morula stage, but the gens showed different expression patterns. During ovarian development, high expression of wnt4a was observed in the ovarian lumen formation and gonium proliferation stage, while wnt4b exhibited strong expression in the early developmental stage of oocytes. Taken together, the present study indicates that the two wnt4 genes are involved in the regulation of ovarian development in the orangespotted grouper. (C) 2015 Elsevier Inc. All rights reserved.