文献类型：期刊文献

英文题名：Vitellogenins and choriogenins are biomarkers for monitoring Oryzias curvinotus juveniles exposed to 17 β - estradiol

作者：Dong, Zhongdian[1];Li, Xueyou[1];Huang, Shunkai[1];Zhang, Ning[1];Guo, Yusong[1];Wang, Zhongduo[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Guangdong Higher Educ Inst, Key Lab Aquaculture South China Sea Aquat Econ An, Zhanjiang, Peoples R China

年份：2020

卷号：236

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY

收录：SCI-EXPANDED(收录号：WOS:000557992800004)、、Scopus(收录号：2-s2.0-85085566407)、WOS

基金：This work was supported by the National Natural Science Foundation of China (41806195, 31972794); Technology Planning Project of Guangdong Province, China (2017A030303075); Special Innovation Projects of Colleges and Universities in Guangdong Province (2018KTSCX087); Innovation Strong School Project of Guangdong Ocean University (230419055), Program for Scientific Research Startup Funds from GDOU.

语种：英文

外文关键词：Oryzias curvinotus; 17 beta- estradiol; RNA-seq; Biomarker

外文摘要：The effect of estrogens on Oryzias curvinotus juveniles were investigated by sequencing the transcriptome of O. curvinotus juveniles exposed to 17 beta - estradiol for 24 h. A total of 69,071,524 and 71,210,528 raw reads were obtained for the control group (NC) and 17 beta - estradiol exposure group (E2), respectively. After de novo assembly, total 133,210 unigenes were identified, and 85,837 unigenes (64.44% of 133,210) were annotated. Analysis of the transcriptome showed that exposure to 2 mu g/L 17 beta - estradiol led to the up-regulation of 19 genes and down-regulation of 18 genes. The eef1b and rps4x was most suitable as controls for quantitative real-time PCR (qPCR) using Reffinder. Different expression genes enrichment analysis found that exposed to 2 mu g/L 17 beta estradiol affected various physiological processes, including spliceosome, phototransduction, amino sugar and nuclear sugar metabolism, hypotaurine metabolism, and renin-angiotensin system, etc. Exposing O. curvinotus juveniles to increasing concentrations of 17 beta - estradiol (2 ng/L, 20 ng/L, 200 ng/L and 2 mu g/L) led to significant up-regulation of vitellogenins (vtgs) and choriogenins (chgs) mRNA expression. The present study is the first highthroughput transcriptome sequencing of O. curvinotus juveniles, which will be useful for future functional analysis of genes related to environmental estrogen exposed, and development of biomarkers.