文献类型：期刊文献

英文题名：Phylogenomic analyses reveal subclass Scuticociliatia as the sister group of subclass Hymenostomatia within class Oligohymenophorea

作者：Feng, Jin-Mei[1,2];Jiang, Chuan-Qi[2];Warren, Alan[3];Tian, Miao[2];Cheng, Jun[2,4];Liu, Guang-Long[2];Xiong, Jie[2];Miao, Wei[2]

机构：[1]Jianghan Univ, Sch Med, Dept Pathogen Biol, Wuhan 430056, Peoples R China;[2]Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China;[3]Nat Hist Museum, Dept Life Sci, London SW7 5BD, England;[4]Guangdong Ocean Univ, Coll Fishery, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China

年份：2015

卷号：90

起止页码：104

外文期刊名：MOLECULAR PHYLOGENETICS AND EVOLUTION

收录：SCI-EXPANDED(收录号：WOS:000357965100009)、、Scopus(收录号：2-s2.0-84930210577)、WOS

基金：The authors are indebted to Prof. Weibo Song (Ocean University of China), Prof. Kuidong Xu (Institute of Oceanology, Chinese Academy of Sciences), and Prof. Tuanyuan Shi (Zhejiang Academy of Agricultural Science) for kindly providing the Pseudocohnilembus persalinus, Paralembus digitiformis, and Colpoda aspera, respectively. This work is supported by the Natural Science Foundation of China (Project No. 31372168).

语种：英文

外文关键词：Oligohymenophorea; Scuticociliatia; Hymenostomatia; Phylogenomics analysis

外文摘要：Scuticociliates and hymenostomes are two groups of the ciliate class Oligohymenophorea, a diverse clade that includes two model genera, Tetrahymena and Paramecium, which have been intensively studied due to their ease of culture and their amenability to a wide range of biochemical and genetic investigations. However, phylogenetic relationships among the subclasses of the Oligohymenophorea, and especially between the Scuticociliatia and Hymenostomatia, are not clearly resolved. Here, we investigate the phylogenetic relationship between the subclasses Scuticociliatia and Hymenostomatia based on omics data. The transcriptomes of five species, comprising four oligohymenophoreans and one colpodean, were sequenced. A supermatrix was constructed for phylogenomic analyses based on 113 genes encoding 43,528 amino acid residues from 26 taxa, including ten representatives of the class Oligohymenophorea. Our phylogenomic analyses revealed that the monophyletic Scuticociliatia is sister to the monophyletic Hymenostomatia, which together form the terminal branch within the monophyletic class Oligohymenophorea. Competing hypotheses for this relationship were rejected by topological tests. Our results provide corroborative evidence for the close relationship between the subclasses Scuticociliatia and Hymenostomatia, justifying the possible use of the model hymenostome T. thermophila as an effective experimental system to study the molecular and cellular biology of the scuticociliates. (C) 2015 Elsevier Inc. All rights reserved.