文献类型：期刊文献

英文题名：Multi-omics unravels energy trade-offs and osmoregulatory strategies in Hyriopsis cumingii under salinity stress

作者：Yang, Xiuyan[1];Sai, Chuchu[1];Huang, Han[1];Tan, Wanqi[1];Lu, Yunzhao[2];Mkuye, Robert[1];Yang, Chuangye[1,4,5,6];Liao, Yongshan[3,4,5];Wang, Qingheng[1,3,4,5,6];Deng, Yuewen[1,3,4,5,6]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Tianjin Fisheries Res Inst, Tianjin 300221, Peoples R China;[3]Guangdong Ocean Univ, Pearl Res Inst, Zhanjiang, Peoples R China;[4]Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Zhanjiang 524088, Peoples R China;[5]Prov Key Lab Aquat Anim Dis Control & Hlth Culture, Zhanjiang 524088, Peoples R China;[6]Guangdong Sci & Innovat Ctr Pearl Culture, Zhanjiang 524088, Peoples R China

年份：2026

卷号：622

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:001753193200001)、、WOS

基金：This research was funded by Youth S&T Talent Support Programme of Guangdong Provincial Association for Science and Technology (Grant no. SKXRC2025389) , Shellfish & Algae Industry Innovation Team of Guangdong Modern Agricultural Technology System (Grant no. 2025CXTD23) , Undergraduate Innovation Team of Guangdong Ocean University (Grant no. CXTD2025001) , the earmarked fund for CARS-49, and the Program for Scientific Research Start-up Funds of Guangdong Ocean University (Grant no. 060302022304) . We thank TopEdit ( www.topeditsci.com ) for linguistic assistance during the preparation of this manuscript.

语种：英文

外文关键词：Freshwater bivalve; Osmoregulation; Energy trade-off; Transcriptomics; Metabolomics

外文摘要：Investigating the salinity tolerance of the freshwater mussel Hyriopsis cumingii is important for enhancing the ecological use of saline waters. This work integrated gill transcriptomics and metabolomics with assays of key hepatopancreatic enzyme activities to characterize the response of H. cumingii to chronic salinity stress (5 PSU) for 40 days). We detected 1011 DEGs and 385 DAMs. Mussels relied on several coordinated mechanisms to mitigate stress. An inorganic ion-regulatory network centered on calcium signaling is triggered; during prolonged exposure, energy allocation shifts by downregulating energetically expensive ion pumps (e.g., Na+/K+-ATPase). An organic osmoregulatory program is engaged that depends on free amino acids (e.g., alanine, taurine), betaine, and glycerol, supported by the marked upregulation of major transporter (Slc1a4, SLC1A3, Abcb1a) and synthase (gadl1) genes. Antioxidant protection is redirected away from the conventional SOD/CAT axis toward a more energy-saving route centered on glutathione metabolism, with GPX2 and chac1/chac2 acting in concert to maintain redox homeostasis. Energy-intensive functions, including muscle contraction (downregulation of Ttn) and biomineralization (downregulation of chs-2, Perlucin), are suppressed, whereas gluconeogenesis (upregulation of Pck1) and lipid mobilization (increased lipase activity) are strengthened. Thus, the salinity response of H. cumingii mounts an integrated response via energy trade-offs and metabolic rewiring. This study sheds new light on the adaptive capacity of H. cumingii across the 1-5 PSU salinity range and has implications for advancing its ecological aquaculture in saline-alkaline waters.