文献类型：期刊文献

英文题名：Novel approach on the evaluation of enzyme-aided alkaline extraction of polysaccharide from Hordeum vulgare husk and molecular insight on the multifunctional scaffold

作者：Chelliah, Ramachandran[1,2,3,4];Park, Chae Rin[1];Park, Se Jin[6];Barathikannan, Kaliyan[1,2,8];Kim, Eun Ji[1];Wei, Shuai[4,5];Sultan, Ghazala[7];Hirad, Abdurahman Hajinur[9];Vijayalakshmi, Selvakumar[1,2];Oh, Deog Hwan[1,2]

机构：[1]Kangwon Natl Univ, Coll Agr & Life Sci, Dept Food Sci & Biotechnol, Chunchon 24341, South Korea;[2]Future F Biotech Co Ltd, Chunchon 24341, South Korea;[3]SIMATS Univ, Saveetha Sch Engn, Sriperumbudur, India;[4]Guangdong Ocean Univ, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Coll Food Sci & Technol, Guangdong Prov Engn Technol Res Ctr Seafood,Guangd, Zhanjiang 524088, Peoples R China;[5]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China;[6]Kangwon Natl Univ, Sch Nat Resources & Environm Sci, Chunchon 24341, South Korea;[7]Aligarh Muslim Univ, Dept Comp Sci, Aligarh 202002, India;[8]Kangwon Natl Univ, Agr & Life Sci Res Inst, Chunchon 24341, South Korea;[9]King Saud Univ, Coll Sci, Dept Bot & Microbiol, POB 2455, Riyadh 11451, Saudi Arabia

年份：2024

卷号：278

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:001297545200001)、、EI(收录号：20243416890065)、WOS

基金：This research was financially supported by the [National Research Foundation of Korea (NRF) ] grant number [2018007551], Brain Korea (B.K.) 21 Plus Project [Grant No. 4299990913942] . The authors extend their appreciation to the Researchers Supporting Project number (RSPD2024R677), King Saud University, Riyadh, Saudi Arabia for financial support.r (B.K.) 21 Plus Project [Grant No. 4299990913942] . The authors extend their appreciation to the Researchers Supporting Project number (RSPD2024R677) , King Saud University, Riyadh, Saudi Arabia for financial support.

语种：英文

外文关键词：Polysaccharides; Xylan; Barley husk; HPLC-RID; FTIR; 1 H NMR; 13 C NMR

外文摘要：Hordeum vulgare husk, a cereal grain, is rich in dietary fiber and prebiotics beneficial for the gut microbiota and host organism. This study investigates the effects of barley husk-derived water-soluble xylan (BH-WSX) on gut homeostasis and the microbiome. We enzymatically extracted BH-WSX and evaluated its prebiotic and antioxidant properties. A 40.0 % (w/v) xylan yield was achieved, with the extracted xylan having a molecular mass of 212.0885 and a xylose to glucuronic acid molar ratio of 6:1. Specialized optical rotation research indicated that the isolated xylan is composed of monomeric sugars such as D-xylose, glucose, and arabinose. Fourier Transform Infrared (FTIR) spectroscopy revealed that the xylan comprises beta (1 -> 4) linked xylose units, randomly substituted with glucose residues, alpha-arabinofuranose, and acetyl groups. Nuclear Magnetic Resonance (NMR) analysis showed that the barley husk extract's backbone is substituted with 4-O-methyl glucuronic acid at the O2 position. Thermogravimetric analysis indicated that WSX exhibits a single sharp peak at 266 degrees C on the Differential Thermal Gravimetry (DTG) curve. Furthermore, a combination of in vitro, in vivo models, and molecular docking analysis elaborated on the anti-adhesion properties of BH-WSX. This study presents a novel approach to utilizing barley husk as an efficient source of functional polysaccharides for food-related industrial applications.