详细信息
文献类型:期刊文献
中文题名:猪笼草组培快繁技术的研究
英文题名:TISSUE CULTURE AND RAPID PROPAGATION OF NEPENTHES MIRABILIS
作者:吕庆芳[1];丰锋[1];李洪波[1]
机构:[1]湛江海洋大学农学院,广东湛江524088
年份:2003
卷号:25
期号:1
起止页码:11
中文期刊名:西南农业大学学报(自然科学版)
外文期刊名:Journal of Southwest Agricultural University
收录:CSTPCD、、北大核心2000、CSCD_E2011_2012、北大核心、CSCD
基金:湛江市科委资助项目
语种:中文
中文关键词:猪笼草;繁殖技术;芽增殖倍数;生根率;组织培养
外文关键词:Nepenthes mirabilis; tissue culture; propagation
中文摘要:以猪笼草(Nepenthesmirabilis)腋芽为试材,通过组织培养进行快繁研究,结果表明,无菌繁殖体系的建立以1/2MS+6-BA1.0(mg/L)+NAA0.05+LH(水解乳蛋白)100为最佳;继代芽的增殖以MS+6-BA1.5+Ad2.0+NAA0.1+30~40g/L蔗糖最好;生根培养用1/2MS+NAA1~2+IBA0.5~1+H3BO350~100mg/L最好。
外文摘要:Axillary buds of Nepenthes mirabilis were used as explants in tissue culture to investigate the optimum conditions for its rapid propagation. The optimum medium appeared to be liquid 1/2MS supplemented with 1 mg/L 6-BA, 0.05 mg/L NAA and 100 mg/L LH for the establishment of virus-free propagation materials; MS supplemented with 1.5 mg/L 6-BA, 4 mg/L Ad, 0.1 mg/L NAA and 30~50 g/L sucrose for the proliferation of the subcultured buds; and 1/2MS supplemented with 1~2 mg/L NAA, 0.5~1 mg/L IBA and 50~100 mg/L H3BO3 for rooting.
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