文献类型：期刊文献

英文题名：High light stress triggers distinct proteomic responses in the marine diatom Thalassiosira pseudonana

作者：Dong, Hong-Po[1,2];Dong, Yue-Lei[1];Cui, Lei[1];Balamurugan, Srinivasan[1];Gao, Jian[1];Lu, Song-Hui[1];Jiang, Tao[1]

机构：[1]Jinan Univ, Res Ctr Harmful Algae & Marine Biol, Key Lab Eutrophicat & Red Tide Prevent Guangdong, Guangzhou 510632, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Sch Ocean & Meteorol, Zhanjiang 524088, Peoples R China

年份：2016

卷号：17

外文期刊名：BMC GENOMICS

收录：SCI-EXPANDED(收录号：WOS:000389842800001)、、WOS

基金：This study was funded by the Guangdong Natural Science Foundation (grant NO. 2015A030313326, S2013010013406), the National Natural Science Foundation of China (grant NO. 41576123, 41176087, 41206126), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA11020304), and General Financial Grant from the China Postdoctoral Science Foundation (Grant No. 2014 M562251).

语种：英文

外文关键词：Thalassiosira pseudonana; iTRAQ labeling; Quantitative proteomics; Light protection

外文摘要：Background: Diatoms are able to acclimate to frequent and large light fluctuations in the surface ocean waters. However, the molecular mechanisms underlying these acclimation responses of diaotms remain elusive. Results: In this study, we investigated the mechanism of high light protection in marine diatom Thalassiosira pseudonana using comparative proteomics in combination with biochemical analyses. Cells treated under high light (800 mu mol photons m(-2)s(-1)) for 10 h were subjected to proteomic analysis. We observed that 143 proteins were differentially expressed under high light treatment. Light-harvesting complex proteins, ROS scavenging systems, photorespiration, lipid metabolism and some specific proteins might be involved in light protection and acclimation of diatoms. Non-photochemical quenching (NPQ) and relative electron transport rate could respond rapidly to varying light intensities. High-light treatment also resulted in increased diadinoxanthin + diatoxanthin content, decreased Fv/Fm, increased triacylglycerol and altered fatty acid composition. Under HL stress, levels of C14:0 and C16:0 increased while C20:5 omega 3 decreased. Conclusions: We demonstrate that T. pseudonana has efficient photoprotective mechanisms to deal with HL stress. De novo synthesis of Ddx/Dtx and lipid accumulation contribute to utilization of the excess energy. Our data will provide new clues for in-depth study of photoprotective mechanisms in diatoms.