文献类型：期刊文献

英文题名：Solid phase synthesis of oxidized sodium alginate-tobramycin conjugate and its application for infected wound healing

作者：Liang, Limei[1];Liu, Tao[2];Ouyang, Qianqian[3];Li, Sidong[1];Li, Chengpeng[1]

机构：[1]Guangdong Ocean Univ, Sch Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Dept Otolaryngol Head & Neck Surg, Guangzhou 510080, Peoples R China;[3]Guangdong Med Univ, Marine Biomed Res Inst, Key Lab Zhanjiang R&D Marine Microbial Resources B, Zhanjiang 524023, Peoples R China

年份：2022

卷号：295

外文期刊名：CARBOHYDRATE POLYMERS

收录：SCI-EXPANDED(收录号：WOS:000831292100001)、、EI(收录号：20222912384801)、Scopus(收录号：2-s2.0-85134314179)、WOS

基金：Acknowledgement This work was financially supported by Natural Science Foundation of Guangdong Province (No. 2020A1515011011 and No. 2019A1515011678) and the Science and Technology Program of Guangzhou City (202002030065) .

语种：英文

外文关键词：Sodium alginate; Antimicrobial activity; Infection; Solid phase synthesis

外文摘要：Although sodium alginate possesses excellent biocompatibility, moisture retention and easy availability, it cannot be directly applied for infected wound treatment. Herein, a solid phase synthesis strategy was proposed to fabricate oxidized sodium alginate-tobramycin conjugate (OSA-TOB) for anti-infection dressing development. C-13 nuclear magnetic resonance spectra indicated that the oxidization process does not change the ratio of beta-D-mannuronic acid (M) / alpha-L-guluronic acid (G) in OSA and the oxidization reaction shows no stereoselectivity. Elemental analysis disclosed that the graft ratio of tobramycin in OSA-TOB is 13.8 %. Antibacterial test indicated that OSA-TOB can effectively inhibit four prevalent pathogenic bacterial S.epidermidis, P. aeruginosa, S. aureus and E. coli via a different antibacterial mechanism compared to the original TOB. Hemolysis and cytotoxicity assays shown that OSA-TOB have superior hemocompatibility and cytocompatibility. Infected wound healing assay shown that the healing rate of OSA-TOB is the highest. Further analysis indicated that OSA-TOB can reduce the local inflammatory response, accelerate the form of epithelium and collagen deposition. In conclusions, OSA-TOB synthesized in solid phase can be potentially applied as a promising anti-infection wound dressing.