文献类型：期刊文献

英文题名：Label-Free Fluorescent Determination of Simian Virus 40 Using Triplex DNA and G-Quadruplex/N-Methyl Mesoporphyrin IX

作者：Wang, Minting[1];Zhao, Liting[1];Wen, Yanmei[1];Wu, Yulian[1];Li, Yubin[1]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China

年份：2021

卷号：54

期号：18

起止页码：2956

外文期刊名：ANALYTICAL LETTERS

收录：SCI-EXPANDED(收录号：WOS:000641828300001)、、Scopus(收录号：2-s2.0-85104695771)、WOS

基金：This work is supported by the Foundation and Applied Foundation Research Joint-Youth Fund project of Guangdong, China (2019A1515110648), the Project of Enhancing School with Innovation of Guangdong Ocean University (2020KZDZX1108), and Project of Enhancing School with Innovation of Guangdong Ocean University (230419054).

语种：英文

外文关键词：Fluorescence; G-quadruplex; N-methyl mesoporphyrin IX; Simian virus 40; triplex DNA

外文摘要：Simian virus 40 (SV40) causes multiple diseases due to its low immunogenicity and strong infectivity. Here, the development of a label-free fluorescent sensor designed to detect double-stranded DNA (dsDNA) via a functionalized molecular beacon (MB) produced using triplex DNA and guanine (G)-quadruplexes is described. A SV40-specific dsDNA sequence was used as the target dsDNA (T-dsDNA). The MB was designed to comprise a G-quadruplex sequence, a complementary homopyrimidine sequence for T-dsDNA, and a partially complementary sequence for G-quadruplex sequence. Addition of T-dsDNA forces the MB to unfold, forming triplex DNA, and releasing the G-quadruplex sequence that binds to N-methyl mesoporphyrin IX (NMM), producing a signal. The G-quadruplex/NMM complex augments the signal at 610 nm and allows qualitative SV40 detection. Under optimal conditions, this strategy demonstrates a low limit of detection (0.4 nM). Compared to traditional biosensors, this sensor is enzyme-free, rapid, selective, sensitive, and was used to determine T-dsDNA in serum, suggesting that this strategy has applications in clinical analysis and for other viruses.