文献类型：期刊文献

中文题名：5种虾虎鱼类线粒体COI基因序列变异及系统进化

英文题名：Sequence Variation and Molecular Phylogeny of Mitochondrial COI Gene Segments from Five Species of Gobiidae Family

作者：廖健[1];张顺[1];龙水生[1];黄承勤[1];郭昱嵩[1];王中铎[1];刘楚吾[1]

机构：[1]广东海洋大学水产学院南海水产经济动物增养殖广东普通高校重点实验室,广东湛江524025

年份：2016

卷号：36

期号：1

起止页码：7

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD

基金：国家自然科学基金项目(31201996);广东省高等学校优秀青年教师培养计划项目(Yq2013089);广东省高等学校优秀青年教师培养计划资助项目(Yq2013091)

语种：中文

中文关键词：虾虎鱼;线粒体;COI基因;系统进化

外文关键词：goby;;Mitochondria;;COI gene;;Phylogenetic

中文摘要：获得雷州半岛红树林海区5 种虾虎鱼稚幼鱼的45 条线粒体COI 基因序列,其T、C、A、G 碱基平均含量分别为29.9%、28.4%、23.9%、17.8%.45 条序列共定义23 个单倍型,其中检测到变异位点184 个,双带缟虾虎鱼（Tridentiger bifasciatus）单倍型比例最高（80.0%）, 湖栖鳍虾虎鱼（Gobiopterus lacustris）单倍型比例最低（18.2%）.Kimura 2-parameter 遗传距离显示,种间遗传距离为0.137 6 - 0.263 5,种内遗传距离为0.000 0 - 0.020 8.NJ 进化树及ML 进化树均表明,聚为5大支系的虾虎鱼类中,阿部氏鲻虾虎鱼（Mugilogobius abei）与诸氏鲻虾虎鱼（M. chulae）亲缘关系最近,湖栖鳍虾虎鱼与其他4 种虾虎鱼的亲缘关系稍远.基于群体内等位基因频率分布的中性检验结果表明,双带缟虾虎鱼（Tridentiger bifasciatus）、小口拟虾虎鱼（ Pseudogobius masago）、阿部氏鲻虾虎鱼、诸氏鲻虾虎鱼4 个群体存在大量低频等位基因位点,而湖栖鳍虾虎鱼群体以中等频率等位基因为主.

外文摘要：45 mitochondrialCOI gene sequences offive kinds of goby juvenilesfrom Mangroves of Leizhou region were obtained,andtheaverage content ofT, C,A, Gwere29.9%, 28.4%, 23.9%, 17.8%, respectively.A total of23 haplotypes were found with 184 variable nucleotide positions,and the haplotype proportionofTridentiger bifasciatuswas thehighest(80.0%), and that ofGobiopterus lacustris was thelowest(18.2%).The inter- and intra-specific genetic distances (Kimura two-parameter, K2P) ranged from 0.1376 to 0.2635, and0.0000 to 0.0208, respectively.Based onNeighbor-Joining and Maximum Likelihood phylogenetic tree,among thegobiesclustered intofive branches, Mugilogobius abeiandM. chulaeare in theclosest relationship,while the relationship between Gobiopterus lacustris and the other fourpopulations is a little far.The neutrality tests indicated thatG. lacustriswasdominated by moderate-frequency allelic loci, while there were a large number of low-frequency allelic loci withinthe other fourpopulations.