文献类型：期刊文献

英文题名：Dietary seaweed (Enteromorpha) polysaccharides improves growth performance involved in regulation of immune responses, intestinal morphology and microbial community in banana shrimp Fenneropenaeus merguiensis

作者：Liu, Wen-Chao[2];Zhou, Shi-Hui[1];Balasubramanian, Balamuralikrishnan[3];Zeng, Fu-Yuan[1];Sun, Cheng-Bo[1];Pang, Huan-Ying[1,4,5]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang 524088, Guangdong, Peoples R China;[3]Sejong Univ, Coll Life Sci, Dept Food Sci & Biotechnol, Seoul 05006, South Korea;[4]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China;[5]Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524025, Peoples R China

年份：2020

卷号：104

起止页码：202

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000564750000010)、、WOS

基金：This study was supported by the Natural Science Foundation of Guangdong Province, China (2018A030307023, 2017A030313174); Talent Research Start-up Project of Guangdong Ocean University, China (R18007, R17103); Innovative Strong School Engineering Youth Talent Project (2017KQNCX090) and Key Platform Projects (2018302) by Deprtment of Education in Guangdong Province, China; National Research Foundation grant (2018R1C1B5086232) funded by Korean Government (MEST), South Korea.

语种：英文

外文关键词：Fenneropenaeus merguiensis; Growth; Immune responses; Intestinal health; Marine polysaccharides

外文摘要：The present study was conducted to evaluate the effects of marine polysaccharides from seaweed Enteromorpha on growth performance, immune responses, intestinal morphology and microbial community in the banana shrimp Fenneropenaeus merguiensis. Two thousand and four hundred juvenile shrimps with an average body weight of 2.18 +/- 0.06 g were fed for 42 d with diets containing different levels of Enteromorpha polysaccharides (EPS): 0 (control), 1, 2 and 3 g/kg as treatment groups, each of group was replicated three times with two hundred shrimps per replicate. Dietary supplementation of 1 g/kg EPS showed a consistent improvement in the final weight, weight gain, average daily gain rate (ADGR) and specific growth rate (SGR) (P < 0.05), while showed a decrease in the feed conversion ratio (FCR) of shrimp (P < 0.05). Besides, the total anti-oxidative capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), lysozyme (Lyz), alkaline phosphatase (ALP), and phenoloxidase (PO) activities in hemolymph were enhanced by dietary supplementation of 1 g/kg EPS (P < 0.05), while it reduced the hemolymph MDA content (P < 0.05). Shrimp fed 1 g/kg EPS supplemented diets up-regulated FmLyz, FmSOD5 and FmCLAP gene expression level of hepatopancreas and gill (P < 0.05), and also improved the intestinal FmLC2, FmLyz, FmSOD5 and FmCLAP gene expression levels (P < 0.05). In addition, shrimp fed diets containing 1 g/kg EPS increased the villus width (P < 0.05) and resulted in a higher villus surface area (P < 0.05). According to 16S rRNA sequencing results, dietary supplementation of 1 g/kg EPS tended to increase the relative abundance of Firmicutes at phylum level (P = 0.07) and decrease the relative abundance of Vibrio at genus level (P = 0.08). There was a significant positive correlation between the relative abundance of Firmicutes and mRNA expression of intestinal immune-related genes (P < 0.05). These findings revealed that dietary 1 g/kg EPS could improve growth performance, enhance nonspecific immunity and modulate intestinal function of banana shrimp F. merguiensis.